Optical detection of harmonic oscillations in fluorescent dye-loaded microbubbles ensonified by ultrasound

Carolyn E. Schutt, Stuart Ibsen, Michael Benchimol, Mark Hsu, Sadik Esener

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A new optical contrast agent has been developed by exposing dye-loaded microbubbles to a rapidly-cooled thermal treatment to homogenize the dye distribution across the surface. Ultrasound causes these microbubbles to oscillate in size which changes the self-quenching efficiency of the dye molecules creating a "blinking" signal. We demonstrate for the first time that these microbubbles can reproducibly generate second, third, and even fourth harmonic fluorescence intensity modulations, in addition to the fundamental frequency of the driving ultrasound. Detecting these harmonic signals could produce a higher signal-to-noise ratio for fluorescence imaging in medical applications by allowing fundamental frequency interference and artifacts to be filtered out.

Original languageEnglish (US)
Pages (from-to)2834-2837
Number of pages4
JournalOptics Letters
Volume40
Issue number12
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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