Optical Control of Lipid Rafts with Photoswitchable Ceramides

James Frank, Henri G. Franquelim, Petra Schwille, Dirk Trauner

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Ceramide is a pro-apoptotic sphingolipid with unique physical characteristics. Often viewed as a second messenger, its generation can modulate the structure of lipid rafts. We prepared three photoswitchable ceramides, ACes, which contain an azobenzene photoswitch allowing for optical control over the N-acyl chain. Using combined atomic force and confocal fluorescence microscopy, we demonstrate that the ACes enable reversible switching of lipid domains in raft-mimicking supported lipid bilayers (SLBs). In the trans-configuration, the ACes localize into the liquid-ordered (Lo) phase. Photoisomerization to the cis-form triggers a fluidification of the Lo domains, as liquid-disordered (Ld) "lakes" are formed within the rafts. Photoisomerization back to the trans-state with blue light stimulates a rigidification inside the Ld phase, as the formation of small Lo domains. These changes can be repeated over multiple cycles, enabling a dynamic spatiotemporal control of the lipid raft structure with light.

Original languageEnglish (US)
Pages (from-to)12981-12986
Number of pages6
JournalJournal of the American Chemical Society
Volume138
Issue number39
DOIs
StatePublished - Oct 5 2016
Externally publishedYes

Fingerprint

Ceramides
Lipids
Liquids
Photoisomerization
Light
Sphingolipids
Lipid Bilayers
Second Messenger Systems
Lakes
Fluorescence Microscopy
Confocal Microscopy
Lipid bilayers
Azobenzene
Confocal microscopy
Fluorescence microscopy

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Optical Control of Lipid Rafts with Photoswitchable Ceramides. / Frank, James; Franquelim, Henri G.; Schwille, Petra; Trauner, Dirk.

In: Journal of the American Chemical Society, Vol. 138, No. 39, 05.10.2016, p. 12981-12986.

Research output: Contribution to journalArticle

Frank, James ; Franquelim, Henri G. ; Schwille, Petra ; Trauner, Dirk. / Optical Control of Lipid Rafts with Photoswitchable Ceramides. In: Journal of the American Chemical Society. 2016 ; Vol. 138, No. 39. pp. 12981-12986.
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