Opposing roles for dopamine and serotonin at presynaptic receptors in the ventral tegmental area

D. L. Cameron, John Williams

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

1. Dopamine D1 and 5HT(1D) receptors are found on the terminals of afferent GABA neurons that synapse on the ventral tegmental area (VTA) dopamine neurons. The role of these receptors in the actions of cocaine was investigated using intracellular recordings in a brain slice preparation. Synaptic potentials were generated in the slice and GABA-mediated inhibitory post-synaptic potentials (IPSP) were identified. 2. Stimulation of dopamine D1 receptors selectively enhanced the GABA(B) IPSP, and this effect was blocked by D1 antagonists. The magnitude of the IPSP was decreased when D1 antagonists were applied in isolation, suggesting tonic D1 receptor stimulation via dendritically released dopamine. 3. Cocaine had an opposite effect and selectively decreased the magnitude of the GABA(B) IPSP. This action was mimicked by 5HT and the 5HT(1D) agonist sumatriptan, and attenuated by the 5HT(1D/2C) antagonist, metergoline. The action of cocaine was also mimicked by the 5HT-releasing agent, fenfluramine, and blocked by pre-incubation of the slice with the 5HT-depleting agent, para-chloroamphetamine. 4. The results of this study suggest that dopamine and 5HT have opposing roles in modulating GABA input into VTA dopamine neurons. The actions of cocaine on this interplay may have implications for understanding its addictive properties.

Original languageEnglish (US)
Pages (from-to)841-845
Number of pages5
JournalClinical and Experimental Pharmacology and Physiology
Volume22
Issue number11
StatePublished - 1995

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Presynaptic Receptors
Synaptic Potentials
Ventral Tegmental Area
Dopamine
Serotonin
Cocaine
gamma-Aminobutyric Acid
Dopaminergic Neurons
p-Chloroamphetamine
Metergoline
Sumatriptan
Fenfluramine
Afferent Neurons
GABAergic Neurons
Dopamine D1 Receptors
Synapses
Brain

Keywords

  • γ-Aminobutyric acid
  • 5HT(1D) receptor
  • Cocaine
  • D receptor
  • Dopamine
  • Electrophysiology
  • Guinea-pig
  • Serotonin
  • Synaptic potential
  • Ventral tegmental area

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Opposing roles for dopamine and serotonin at presynaptic receptors in the ventral tegmental area. / Cameron, D. L.; Williams, John.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 22, No. 11, 1995, p. 841-845.

Research output: Contribution to journalArticle

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