Opioid receptor internalization contributes to dermorphin-mediated antinociception

T. A. Macey, Susan Ingram, E. N. Bobeck, D. M. Hegarty, Sue Aicher, Seksiri Arttamangkul, M. M. Morgan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Microinjection of opioids into the ventrolateral periaqueductal gray (vlPAG) produces antinociception in part by binding to mu-opioid receptors (MOPrs). Although both high and low efficacy agonists produce antinociception, low efficacy agonists such as morphine produce limited MOPr internalization suggesting that MOPr internalization and signaling leading to antinociception are independent. This hypothesis was tested in awake, behaving rats using DERM-A594, a fluorescently labeled dermorphin analog, and internalization blockers. Microinjection of DERM-A594 into the vlPAG produced both antinociception and internalization of DERM-A594. Administration of the irreversible opioid receptor antagonist beta-chlornaltrexamine (beta-CNA) prior to DERM-A594 microinjection reduced both the antinociceptive effect and the number of DERM-A594 labeled cells demonstrating that both effects are opioid receptor-mediated. Pretreatment with the internalization blockers dynamin dominant-negative inhibitory peptide (dynamin-DN) and concanavalinA (ConA) attenuated both DERM-A594 internalization and antinociception. Microinjection of dynamin-DN and ConA also decreased the antinociceptive potency of the unlabeled opioid agonist dermorphin when microinjected into the vlPAG as demonstrated by rightward shifts in the dose-response curves. In contrast, administration of dynamin-DN had no effect on the antinociceptive effect of microinjecting the GABAA receptor antagonist bicuculline into the vlPAG. The finding that dermorphin-induced antinociception is attenuated by blocking receptor internalization indicates that key parts of opioid receptor-mediated signaling depend on internalization.

Original languageEnglish (US)
Pages (from-to)543-550
Number of pages8
JournalNeuroscience
Volume168
Issue number2
DOIs
StatePublished - Jun 2010

Fingerprint

Periaqueductal Gray
Microinjections
Opioid Receptors
Dynamins
mu Opioid Receptor
Peptides
Opioid Analgesics
GABA-A Receptor Antagonists
Bicuculline
Narcotic Antagonists
Morphine
dermorphin

Keywords

  • Analgesia
  • Endocytosis
  • Opiate
  • Pain modulation
  • Periaqueductal gray

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Opioid receptor internalization contributes to dermorphin-mediated antinociception. / Macey, T. A.; Ingram, Susan; Bobeck, E. N.; Hegarty, D. M.; Aicher, Sue; Arttamangkul, Seksiri; Morgan, M. M.

In: Neuroscience, Vol. 168, No. 2, 06.2010, p. 543-550.

Research output: Contribution to journalArticle

Macey, T. A. ; Ingram, Susan ; Bobeck, E. N. ; Hegarty, D. M. ; Aicher, Sue ; Arttamangkul, Seksiri ; Morgan, M. M. / Opioid receptor internalization contributes to dermorphin-mediated antinociception. In: Neuroscience. 2010 ; Vol. 168, No. 2. pp. 543-550.
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