Opioid inhibition in locus coeruleus

R. A. Travagli, T. V. Dunwiddie, John Williams

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

1. Inhibition of locus coeruleus (LC) neurons by opioids is mediated by hyperpolarization associated with an increase in potassium conductance. However, opioids caused an outward current even at potentials more negative than the potassium equilibrium potential in the majority of LC neurons recorded in brain slices cut in the horizontal plane. 2. Whole cell and intracellular recording were made from LC neurons in the slice preparation and currents were measured with a switch clamp amplifier. 3. Local application of [Met]5enkephalin (ME) by iontophoresis resulted in two different effects depending on the site of application. Iontophoresis of ME close to the cell caused an outward current that reversed polarity near the potassium equilibrium potential. Whereas application at a distance >200 μm resulted in an outward current that did not reverse polarity even at strongly negative potentials. 4. When potassium conductances were blocked with internal Cs and/or external Ba, the opioid current was reduced to

Original languageEnglish (US)
Pages (from-to)519-528
Number of pages10
JournalJournal of Neurophysiology
Volume74
Issue number2
StatePublished - 1995

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Locus Coeruleus
Opioid Analgesics
Potassium
Iontophoresis
Neurons
Patch-Clamp Techniques
Brain

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Travagli, R. A., Dunwiddie, T. V., & Williams, J. (1995). Opioid inhibition in locus coeruleus. Journal of Neurophysiology, 74(2), 519-528.

Opioid inhibition in locus coeruleus. / Travagli, R. A.; Dunwiddie, T. V.; Williams, John.

In: Journal of Neurophysiology, Vol. 74, No. 2, 1995, p. 519-528.

Research output: Contribution to journalArticle

Travagli, RA, Dunwiddie, TV & Williams, J 1995, 'Opioid inhibition in locus coeruleus', Journal of Neurophysiology, vol. 74, no. 2, pp. 519-528.
Travagli RA, Dunwiddie TV, Williams J. Opioid inhibition in locus coeruleus. Journal of Neurophysiology. 1995;74(2):519-528.
Travagli, R. A. ; Dunwiddie, T. V. ; Williams, John. / Opioid inhibition in locus coeruleus. In: Journal of Neurophysiology. 1995 ; Vol. 74, No. 2. pp. 519-528.
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