Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhesus monkeys

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    4 Citations (Scopus)

    Abstract

    Purpose: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro. Methods: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n = 91; >0.5 mm; n = 5 monkeys) was evaluated after 34 h of culture in the absence (control) or presence of hCG. Results: Of the total cohort (n = 91), 49 % of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p <0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p <0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p <0.05) P4 and AMH compared to SAFs containing GV oocytes (p <0.05). Follicles ≥1 mm produced more (p <0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p <0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes. Conclusions: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality.

    Original languageEnglish (US)
    Pages (from-to)353-359
    Number of pages7
    JournalJournal of Assisted Reproduction and Genetics
    Volume30
    Issue number3
    DOIs
    StatePublished - Mar 2013

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    In Vitro Oocyte Maturation Techniques
    Macaca mulatta
    Oocytes
    Hormones
    Anti-Mullerian Hormone
    Gonadotropins
    Meiosis
    Antral
    Metaphase
    Ovarian Follicle

    Keywords

    • E2
    • Oocyte maturation
    • P4 and AMH
    • Rhesus monkey
    • Small antral follicle

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Reproductive Medicine
    • Developmental Biology
    • Genetics
    • Genetics(clinical)

    Cite this

    @article{facd7115ffef472db19efc0fa2558123,
    title = "Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhesus monkeys",
    abstract = "Purpose: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro. Methods: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n = 91; >0.5 mm; n = 5 monkeys) was evaluated after 34 h of culture in the absence (control) or presence of hCG. Results: Of the total cohort (n = 91), 49 {\%} of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p <0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p <0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p <0.05) P4 and AMH compared to SAFs containing GV oocytes (p <0.05). Follicles ≥1 mm produced more (p <0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p <0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes. Conclusions: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality.",
    keywords = "E2, Oocyte maturation, P4 and AMH, Rhesus monkey, Small antral follicle",
    author = "Peluffo, {Marina C.} and Jon Hennebold and Richard Stouffer and Mary Zelinski",
    year = "2013",
    month = "3",
    doi = "10.1007/s10815-013-9937-9",
    language = "English (US)",
    volume = "30",
    pages = "353--359",
    journal = "Journal of Assisted Reproduction and Genetics",
    issn = "1058-0468",
    publisher = "Springer New York",
    number = "3",

    }

    TY - JOUR

    T1 - Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhesus monkeys

    AU - Peluffo, Marina C.

    AU - Hennebold, Jon

    AU - Stouffer, Richard

    AU - Zelinski, Mary

    PY - 2013/3

    Y1 - 2013/3

    N2 - Purpose: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro. Methods: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n = 91; >0.5 mm; n = 5 monkeys) was evaluated after 34 h of culture in the absence (control) or presence of hCG. Results: Of the total cohort (n = 91), 49 % of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p <0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p <0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p <0.05) P4 and AMH compared to SAFs containing GV oocytes (p <0.05). Follicles ≥1 mm produced more (p <0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p <0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes. Conclusions: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality.

    AB - Purpose: The small antral follicles (SAFs) from the ovarian medulla can be a potential source of oocytes for infertility patients, but little is known about their ability to yield mature oocytes. This study evaluated the response of these SAFs to a stimulatory bolus of human corionic gonadotropin (hCG) in vitro. Methods: Oocyte nuclear maturation and hormone production (estradiol [E2], progesterone [P4]), antimullerian hormone [AMH]) by individual intact SAFs (n = 91; >0.5 mm; n = 5 monkeys) was evaluated after 34 h of culture in the absence (control) or presence of hCG. Results: Of the total cohort (n = 91), 49 % of SAFs contained degenerating oocytes. The percentage of healthy oocytes able to reinitiate meiosis to the metaphase I (MI) and MII was greater (p <0.05) after hCG compared to controls. E2, P4 and AMH levels were higher (p <0.05) in SAF cultures containing germinal vesicle (GV) oocytes compared to those with MII oocytes regardless of hCG exposure. SAF with MI oocytes produced more E2, but less (p <0.05) P4 and AMH compared to SAFs containing GV oocytes (p <0.05). Follicles ≥1 mm produced more (p <0.05) E2, whereas follicle diameter did not correlate with P4 or AMH levels. Only P4 increased (p <0.05) in response to hCG, regardless of follicle size or oocyte maturity. SAFs containing degenerating oocytes produced similar levels of E2, P4 and AMH compared to SAFs containing healthy oocytes. Conclusions: These data indicate, for the first time, that oocytes within primate SAFs can reinitiate meiosis in vitro in the absence of hCG, but nuclear maturation is enhanced in SAFs cultured with hCG. Oocyte nuclear maturation within SAFs in is associated with decreased E2, P4 and AMH levels. Furthermore, hormone content within the culture media does not necessarily reflect oocyte quality.

    KW - E2

    KW - Oocyte maturation

    KW - P4 and AMH

    KW - Rhesus monkey

    KW - Small antral follicle

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    U2 - 10.1007/s10815-013-9937-9

    DO - 10.1007/s10815-013-9937-9

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    AN - SCOPUS:84876298045

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    JO - Journal of Assisted Reproduction and Genetics

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    SN - 1058-0468

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