Oocyte age and nuclear donor cell type affect the technical efficiency of somatic cloning in rabbits

Rita P. Cervera, Fernando García-Ximénez

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The present study in rabbits compared, in the first experiment, the effect of two commonly used oocyte ages, 13 h and 17 h after ovulation induction treatment, on the technical efficiency of somatic nuclear transfer steps, using fresh cumulus cells as nuclear donors. Recently ovulated metaphase II oocytes (13 h) showed higher fusion (13 h: 83% vs 17 h: 67%, p < 0.05) and in vitro development rates than in vivo slightly aged metaphase II oocytes (morula, 13 h: 74% vs 17 h: 25%, p < 0.05; blastocyst, 13 h: 16% vs 17 h: 8%; p < 0.05). In contrast, activation rate was higher in the 17 h group (13 h: 45% vs 17 h: 67%; p < 0.05). In a second experiment, using recently ovulated oocytes (13 h) as recipients, two donor cell types (from primary cultures of either cumulus cells or fetal fibroblasts) were tested to evaluate their effects on the efficiencies of the different technical steps of somatic nuclear transfer procedure. A better fusion rate was obtained when fetal fibroblasts were used as nuclear donors (cumulus cells: 45% vs fetal fibroblasts: 67%, p < 0.05). No statistically significant differences were detected in cleavage rate regardless of the cell type used (cumulus cells: 44% vs fetal fibroblasts: 60%, p > 0.05). However, in vitro development to morula (cumulus cells: 41% vs fetal fibroblasts: 14%, p < 0.05) and to blastocyst stage (cumulus cells: 27% vs fetal fibroblasts: 3%, p < 0.05) were different between cell types.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalZygote
Volume11
Issue number2
DOIs
StatePublished - May 2003
Externally publishedYes

Keywords

  • Cumulus cells
  • Fetal fibroblasts
  • Oocyte ageing
  • Rabbit
  • Somatic nuclear transfer

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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