Ontogeny of serotonin and serotonin2A receptors in rat auditory cortex

Gregory J. Basura, Atheir I. Abbas, Heather O'Donohue, Jean M. Lauder, Bryan L. Roth, Paul D. Walker, Paul B. Manis

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Maturation of the mammalian cerebral cortex is, in part, dependent upon multiple coordinated afferent neurotransmitter systems and receptor-mediated cellular linkages during early postnatal development. Given that serotonin (5-HT) is one such system, the present study was designed to specifically evaluate 5-HT tissue content as well as 5-HT2A receptor protein levels within the developing auditory cortex (AC). Using high performance liquid chromatography (HPLC), 5-HT and the metabolite, 5-hydroxyindoleacetic acid (5-HIAA), was measured in isolated AC, which demonstrated a developmental dynamic, reaching young adult levels early during the second week of postnatal development. Radioligand binding of 5-HT2A receptors with the 5-HT2A/2C receptor agonist, 125I-DOI ((+/-)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane HCl; in the presence of SB206553, a selective 5-HT2C receptor antagonist, also demonstrated a developmental trend, whereby receptor protein levels reached young adult levels at the end of the first postnatal week (P8), significantly increased at P10 and at P17, and decreased back to levels not significantly different from P8 thereafter. Immunocytochemical labeling of 5-HT2A receptors and confocal microscopy revealed that 5-HT2A receptors are largely localized on layer II/III pyramidal cell bodies and apical dendrites within AC. When considered together, the results of the present study suggest that 5-HT, likely through 5-HT2A receptors, may play an important role in early postnatal AC development.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalHearing Research
Issue number1-2
StatePublished - Oct 2008
Externally publishedYes


  • Auditory cortex
  • Autoradiography
  • High performance liquid chromatography
  • Immunocytochemistry
  • Serotonin
  • Serotonin receptors

ASJC Scopus subject areas

  • Sensory Systems


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