ON THE SPECIFICITY OF TRYPSIN (EC 3.4.4.4) OF NERVE ENDING PARTICLES TO INHIBIT NOREPINEPHRINE TRANSPORT

Barbara A. Hitzemann, R. J. Hitzemann, H. H. Loh

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The Na+ and energy dependent uptake of norepinephrine into cortical rat brain homogenates or purified nerve ending particles (NEP) is reduced by prior trypsin treatment. In contrast, the uptake of dopamine, serotonin, choline and γ‐aminobutyric acid is markedly less sensitive to the effect of trypsin. Kinetic analyses indicate that the trypsin‐induced decrease of norepinephrine uptake is non‐competitive. In the dose range studied, trypsin did not appreciably alter the protein content or morphology of NEP. However, in a dose related fashion, trypsin decreased the glycoprotein content of NEP measured as the loss of protein bound N‐acetylneuraminic acid.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalJournal of neurochemistry
Volume24
Issue number2
DOIs
StatePublished - Feb 1975

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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