TY - JOUR
T1 - ON THE SPECIFICITY OF TRYPSIN (EC 3.4.4.4) OF NERVE ENDING PARTICLES TO INHIBIT NOREPINEPHRINE TRANSPORT
AU - Hitzemann, Barbara A.
AU - Hitzemann, R. J.
AU - Loh, H. H.
PY - 1975/2
Y1 - 1975/2
N2 - The Na+ and energy dependent uptake of norepinephrine into cortical rat brain homogenates or purified nerve ending particles (NEP) is reduced by prior trypsin treatment. In contrast, the uptake of dopamine, serotonin, choline and γ‐aminobutyric acid is markedly less sensitive to the effect of trypsin. Kinetic analyses indicate that the trypsin‐induced decrease of norepinephrine uptake is non‐competitive. In the dose range studied, trypsin did not appreciably alter the protein content or morphology of NEP. However, in a dose related fashion, trypsin decreased the glycoprotein content of NEP measured as the loss of protein bound N‐acetylneuraminic acid.
AB - The Na+ and energy dependent uptake of norepinephrine into cortical rat brain homogenates or purified nerve ending particles (NEP) is reduced by prior trypsin treatment. In contrast, the uptake of dopamine, serotonin, choline and γ‐aminobutyric acid is markedly less sensitive to the effect of trypsin. Kinetic analyses indicate that the trypsin‐induced decrease of norepinephrine uptake is non‐competitive. In the dose range studied, trypsin did not appreciably alter the protein content or morphology of NEP. However, in a dose related fashion, trypsin decreased the glycoprotein content of NEP measured as the loss of protein bound N‐acetylneuraminic acid.
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U2 - 10.1111/j.1471-4159.1975.tb11883.x
DO - 10.1111/j.1471-4159.1975.tb11883.x
M3 - Article
C2 - 1113109
AN - SCOPUS:0016468242
SN - 0022-3042
VL - 24
SP - 323
EP - 330
JO - Journal of neurochemistry
JF - Journal of neurochemistry
IS - 2
ER -