On the relationships in rhesus macaques between chronic ethanol consumption and the brain transcriptome

Ovidiu Iancu, Alexander Colville, Nicole A R Walter, Priscila Darakjian, Denesa L. Oberbeck, James B. Daunais, Christina Zheng, Robert Searles, Shannon McWeeney, Kathleen (Kathy) Grant, Robert Hitzemann

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This is the first description of the relationship between chronic ethanol self-administration and the brain transcriptome in a non-human primate (rhesus macaque). Thirty-one male animals self-administered ethanol on a daily basis for over 12 months. Gene transcription was quantified with RNA-Seq in the central nucleus of the amygdala (CeA) and cortical Area 32. We constructed coexpression and cosplicing networks, and we identified areas of preservation and areas of differentiation between regio2uuuu w ns and network types. Correlations between intake and transcription included largely distinct gene sets and annotation categories across brain regions and between expression and splicing; positive and negative correlations were also associated with distinct annotation groups. Membrane, synaptic and splicing annotation categories were over-represented in the modules (gene clusters) enriched in positive correlations (CeA); our cosplicing analysis further identified the genes affected only at the exon inclusion level. In the CeA coexpression network, we identified Rab6b, Cdk18 and Igsf21 among the intake-correlated hubs, while in the Area 32, we identified a distinct hub set that included Ppp3r1 and Myeov2. Overall, the data illustrate that excessive ethanol self-administration is associated with broad expression and splicing mechanisms that involve membrane and synapse genes.

Original languageEnglish (US)
JournalAddiction Biology
DOIs
StateAccepted/In press - 2017

Fingerprint

Macaca mulatta
Transcriptome
Self Administration
Ethanol
Brain
Genes
Molecular Sequence Annotation
Synaptic Membranes
Multigene Family
Synapses
Primates
Exons
RNA
Membranes
Central Amygdaloid Nucleus

Keywords

  • Addiction
  • Cosplicing
  • Ethanol
  • Network analysis
  • Non-human primate
  • RNA-Seq

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology
  • Psychiatry and Mental health

Cite this

On the relationships in rhesus macaques between chronic ethanol consumption and the brain transcriptome. / Iancu, Ovidiu; Colville, Alexander; Walter, Nicole A R; Darakjian, Priscila; Oberbeck, Denesa L.; Daunais, James B.; Zheng, Christina; Searles, Robert; McWeeney, Shannon; Grant, Kathleen (Kathy); Hitzemann, Robert.

In: Addiction Biology, 2017.

Research output: Contribution to journalArticle

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