Oligomerization of the response regulator come from streptococcus mutans is affected by phosphorylation

David C I Hung, Jennifer S. Downey, Jens Kreth, Fengxia Qi, Wenyuan Shi, Dennis G. Cvitkovitch, Steven D. Goodman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously characterized the interactions of the response regulator ComE from Streptococcus mutans and DNA binding sites through DNase I footprinting and electrophoretic mobility shift assay analysis. Since response regulator functions are often affected by their phosphorylation state, we investigated how phosphorylation affects the biochemical function of ComE. Unlike many response regulators, we found that the phosphorylation state of ComE does not likely play a role in DNA binding affinity but rather seems to induce the formation of an oligomeric form of the protein. The role of this oligomerization state for ComE function is discussed.

Original languageEnglish (US)
Pages (from-to)1127-1135
Number of pages9
JournalJournal of Bacteriology
Volume194
Issue number5
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Streptococcus mutans
Phosphorylation
Deoxyribonuclease I
DNA
Electrophoretic Mobility Shift Assay
Binding Sites
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Hung, D. C. I., Downey, J. S., Kreth, J., Qi, F., Shi, W., Cvitkovitch, D. G., & Goodman, S. D. (2012). Oligomerization of the response regulator come from streptococcus mutans is affected by phosphorylation. Journal of Bacteriology, 194(5), 1127-1135. https://doi.org/10.1128/JB.06565-11

Oligomerization of the response regulator come from streptococcus mutans is affected by phosphorylation. / Hung, David C I; Downey, Jennifer S.; Kreth, Jens; Qi, Fengxia; Shi, Wenyuan; Cvitkovitch, Dennis G.; Goodman, Steven D.

In: Journal of Bacteriology, Vol. 194, No. 5, 01.03.2012, p. 1127-1135.

Research output: Contribution to journalArticle

Hung, David C I ; Downey, Jennifer S. ; Kreth, Jens ; Qi, Fengxia ; Shi, Wenyuan ; Cvitkovitch, Dennis G. ; Goodman, Steven D. / Oligomerization of the response regulator come from streptococcus mutans is affected by phosphorylation. In: Journal of Bacteriology. 2012 ; Vol. 194, No. 5. pp. 1127-1135.
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