Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse

Cristina Guatimosim, Court Hull, Henrique Von Gersdorff, Marco A M Prado

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Protein phosphorylation plays an essential role in regulating synaptic transmission and plasticity. However, regulation of vesicle trafficking towards and away from the plasma membrane is poorly understood. Furthermore, the extent to which phosphorylation modulates ribbon-type synapses is unknown. Using the phosphatase inhibitor okadaic acid (OA), we investigated the influence of persistent phosphorylation on vesicle cycling in goldfish bipolar cells. We followed uptake of FM1-43 during vesicle recycling in control and OA-treated cells. FM1-43 fluorescence spread to the center of control synaptic terminals after depolarization elicited Ca2+ influx. However, OA (1-50 nM) impaired this spatial spread of FM1-43 in a dose-dependent manner. Capacitance measurements revealed that OA (50 nM) did not modify either the amount or kinetics of exocytosis and endocytosis evoked by depolarizing pulses. The extremely low concentrations of OA (1-5 nM) sufficient to observe the inhibition of vesicle mobility implicate phosphatase 2A (PP2A) as a major regulator of vesicle trafficking after endocytosis. These results contrast with those at the neuromuscular junction where OA enhances lateral movement of vesicles between distinct vesicle clusters. Thus, our results suggest that phosphatases regulate vesicle translocation at ribbon synapses in a different manner than conventional active zones.

Original languageEnglish (US)
Pages (from-to)1047-1057
Number of pages11
JournalJournal of Neurochemistry
Volume82
Issue number5
DOIs
StatePublished - Sep 2002

Fingerprint

Okadaic Acid
Synaptic Vesicles
Synapses
Phosphorylation
Phosphoric Monoester Hydrolases
Endocytosis
Goldfish
Neuronal Plasticity
Capacitance measurement
Neuromuscular Junction
Exocytosis
Depolarization
Presynaptic Terminals
Recycling
Cell membranes
Synaptic Transmission
Plasticity
Fluorescence
Cells
Cell Membrane

Keywords

  • Capacitance
  • FM1-43
  • Phosphatase
  • Phosphorylation
  • Recycling
  • Ribbon synapse

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse. / Guatimosim, Cristina; Hull, Court; Von Gersdorff, Henrique; Prado, Marco A M.

In: Journal of Neurochemistry, Vol. 82, No. 5, 09.2002, p. 1047-1057.

Research output: Contribution to journalArticle

Guatimosim, Cristina ; Hull, Court ; Von Gersdorff, Henrique ; Prado, Marco A M. / Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse. In: Journal of Neurochemistry. 2002 ; Vol. 82, No. 5. pp. 1047-1057.
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