Odorant-binding protein: Characterization of ligand binding

Jonathan Pevsner, Vivian Hou, Adele M. Snowman, Solomon H. Snyder

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

We have characterized the odorant binding properties of purified bovine odorant-binding protein (OBP) using as a ligand [3H]3,7-dimethyloctan-1-ol ([3H] DMO). A broad variety of odorants, including terpenes, aldehydes, esters, and musks, bind to OBP with affinities of 0.2 to 100 μM. Odorant affinities for OBP correlate most closely with their stimulation of an odorant-sensitive adenylyl cyclase as well as hydrophobicity. We also measured the kinetics of binding for the ligands, [3H]DMO and 2-isobutyl-3-[3H]methoxypyrazine. Dissociation of both is markedly accelerated in the presence of excess unlabeled ligand. Competition curves of displacers for [3H]DMO binding are shallow, and saturation binding isotherms for 3H-odorants are curvilinear. These kinetic and equilibrium binding properties suggest that OBP interactions with odorant ligands are negatively cooperative.

Original languageEnglish (US)
Pages (from-to)6118-6125
Number of pages8
JournalJournal of Biological Chemistry
Volume265
Issue number11
StatePublished - 1990
Externally publishedYes

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Ligands
Kinetics
Terpenes
Hydrophobicity
Adenylyl Cyclases
Aldehydes
Isotherms
Esters
Hydrophobic and Hydrophilic Interactions
odorant-binding protein
Odorants
3,7-dimethyloctan-1-ol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pevsner, J., Hou, V., Snowman, A. M., & Snyder, S. H. (1990). Odorant-binding protein: Characterization of ligand binding. Journal of Biological Chemistry, 265(11), 6118-6125.

Odorant-binding protein : Characterization of ligand binding. / Pevsner, Jonathan; Hou, Vivian; Snowman, Adele M.; Snyder, Solomon H.

In: Journal of Biological Chemistry, Vol. 265, No. 11, 1990, p. 6118-6125.

Research output: Contribution to journalArticle

Pevsner, J, Hou, V, Snowman, AM & Snyder, SH 1990, 'Odorant-binding protein: Characterization of ligand binding', Journal of Biological Chemistry, vol. 265, no. 11, pp. 6118-6125.
Pevsner, Jonathan ; Hou, Vivian ; Snowman, Adele M. ; Snyder, Solomon H. / Odorant-binding protein : Characterization of ligand binding. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 11. pp. 6118-6125.
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