Ocular malformations of Xenopus laevis exposed to nickel during embryogenesis

O. Hauptman, Daniel Albert, M. C. Plowman, S. M. Hopfer, F. W. Sunderman

Research output: Contribution to journalArticle

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Abstract

The pathogenesis of eye anomalies induced by exposure to Ni2+ (as nickel chloride) during embryogenesis was studied in the frog, Xenopus laevis. Eyes of control and Ni2+-exposed tadpoles were examined without staining using a dissecting microscope, by light microscopy of histological sections, and by electron microscopy. The ocular abnormalities of Ni2+-exposed tadpoles included (a) microphthalmia, (b) hypopigmentation, (c) hernias and cysts of the choroid and retina, and (d) iris coloboma; cataracts were uncommon. The pathogenesis of the ocular lesions appears to involve diffuse or focal dysplasia and loss of the retinal pigment epithelium, with dystrophy of photoreceptor outer segments and protrusion of neuroretina through gaps in the pigment epithelium. This study confirms that Ni2+ is a potent ocular teratogen for Xenopus embryos and points to the retinal pigment epithelium as a primary cellular target for Ni2+-induced embryotoxicity.

Original languageEnglish (US)
Pages (from-to)397-406
Number of pages10
JournalAnnals of Clinical and Laboratory Science
Volume23
Issue number6
StatePublished - Jan 1 1993
Externally publishedYes

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Retinal Pigments
Xenopus laevis
Nickel
Embryonic Development
Teratogens
Retinal Pigment Epithelium
Pigments
Electron microscopy
Optical microscopy
Larva
Microscopes
Eye Abnormalities
Hypopigmentation
Coloboma
Microphthalmos
Hernia
Xenopus
Anura
Cataract
Cysts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Hauptman, O., Albert, D., Plowman, M. C., Hopfer, S. M., & Sunderman, F. W. (1993). Ocular malformations of Xenopus laevis exposed to nickel during embryogenesis. Annals of Clinical and Laboratory Science, 23(6), 397-406.

Ocular malformations of Xenopus laevis exposed to nickel during embryogenesis. / Hauptman, O.; Albert, Daniel; Plowman, M. C.; Hopfer, S. M.; Sunderman, F. W.

In: Annals of Clinical and Laboratory Science, Vol. 23, No. 6, 01.01.1993, p. 397-406.

Research output: Contribution to journalArticle

Hauptman, O, Albert, D, Plowman, MC, Hopfer, SM & Sunderman, FW 1993, 'Ocular malformations of Xenopus laevis exposed to nickel during embryogenesis', Annals of Clinical and Laboratory Science, vol. 23, no. 6, pp. 397-406.
Hauptman, O. ; Albert, Daniel ; Plowman, M. C. ; Hopfer, S. M. ; Sunderman, F. W. / Ocular malformations of Xenopus laevis exposed to nickel during embryogenesis. In: Annals of Clinical and Laboratory Science. 1993 ; Vol. 23, No. 6. pp. 397-406.
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