Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle

Lucy H. Clerk, Michelle A. Vincent, Linda A. Jahn, Zhenqi Liu, Jonathan Lindner, Eugene J. Barrett

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

We have previously shown that skeletal muscle capillaries are rapidly recruited by physiological doses of insulin in both humans and animals. This facilitates glucose and insulin delivery to muscle, thus augmenting glucose uptake. In obese rats, both insulin-mediated microvascular recruitment and glucose uptake are diminished; however, this action of insulin has not been studied in obese humans. Here we used contrast ultrasound to measure microvascular blood volume (MBV) (an index of microvascular recruitment) in the forearm flexor muscles of lean and obese adults before and after a 120-min euglycemic-hyperinsulinemic (1 mU·min-1·kg -1) clamp. We also measured brachial artery flow, fasting lipid profile, and anthropomorphic variables. Fasting plasma glucose (5.4 ± 0.1 vs. 5.1 ± 0.1 mmol/l, P = 0.05), insulin (79 ± 11 vs. 38 ± 6 pmol/l, P = 0.003), and percent body fat (44 ± 2 vs. 25 ± 2%, P = 0.001) were higher in the obese than the lean adults. After 2 h of insulin infusion, whole-body glucose infusion rate was significantly lower in the obese versus lean group (19.3 ± 3.2 and 37.4 ± 2.6 μmol· min-1·kg-1 respectively, P <0.001). Compared with baseline, insulin increased MBV in the lean (18.7 ± 3.3 to 25.0 ± 4.1, P = 0.019) but not in the obese group (20.4 ± 3.6 to 18.8 ± 3.8, NS). Insulin increased brachial artery diameter and flow in the lean but not in the obese group. We observed a significant, negative correlation between MBV and ΔBMI (R = -0.482, P = 0.027) in response to insulin. In conclusion, obesity eliminated the insulin-stimulated muscle microvascular recruitment and increased brachial artery blood flow seen in lean individuals.

Original languageEnglish (US)
Pages (from-to)1436-1442
Number of pages7
JournalDiabetes
Volume55
Issue number5
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Forearm
Obesity
Insulin
Muscles
Brachial Artery
Glucose
Blood Volume
Fasting
Adipose Tissue
Skeletal Muscle
Lipids

Keywords

  • MBV
  • MFV, microvascular flow velocity
  • Microvascular blood volume

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Clerk, L. H., Vincent, M. A., Jahn, L. A., Liu, Z., Lindner, J., & Barrett, E. J. (2006). Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle. Diabetes, 55(5), 1436-1442. https://doi.org/10.2337/db05-1373

Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle. / Clerk, Lucy H.; Vincent, Michelle A.; Jahn, Linda A.; Liu, Zhenqi; Lindner, Jonathan; Barrett, Eugene J.

In: Diabetes, Vol. 55, No. 5, 2006, p. 1436-1442.

Research output: Contribution to journalArticle

Clerk, LH, Vincent, MA, Jahn, LA, Liu, Z, Lindner, J & Barrett, EJ 2006, 'Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle', Diabetes, vol. 55, no. 5, pp. 1436-1442. https://doi.org/10.2337/db05-1373
Clerk, Lucy H. ; Vincent, Michelle A. ; Jahn, Linda A. ; Liu, Zhenqi ; Lindner, Jonathan ; Barrett, Eugene J. / Obesity blunts insulin-mediated microvascular recruitment in human forearm muscle. In: Diabetes. 2006 ; Vol. 55, No. 5. pp. 1436-1442.
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