O-glycosylation regulates autolysis of cellular membrane type-1 matrix metalloproteinase (MT1-MMP)

Albert G. Remacle, Alexei V. Chekanov, Vladislav S. Golubkov, Alexei Y. Savinov, Dmitri Rozanov, Alex Y. Strongin

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

MT1-MMP is a key enzyme in cancer cell invasion and metastasis. The activity of cellular MT1-MMP is regulated by furin-like proprotein convertases, TIMPs, shedding, autoproteolysis, dimerization, exocytosis, endocytosis, and recycling. Our data demonstrate that, in addition to these already known mechanisms, MT1-MMPis regulated by O-glycosylation of its hinge region. Insignificant autolytic degradation is characteristic for naturally expressed, glycosylated, MT1-MMP. In turn, extensive autolytic degradation, which leads to the inactivation of the protease and the generation of its C-terminal membrane-tethered degraded species, is a feature of overexpressed MT1-MMP. We have determined that incomplete glycosylation stimulates extensive autocatalytic degradation and self-inactivation of MT1-MMP. Self-proteolysis commences during the secretory process of MT1-MMP through the cell compartment to the plasma membrane. The strongly negatively charged sialic acid is the most important functional moiety of the glycopart of MT1-MMP. We hypothesize that sialic acid of the O-glycosylation cassette restricts the access of the catalytic domain to the hinge region and to the autolytic cleavage site and protects MT1-MMP from autolysis. Overall, our results point out that there is a delicate balance between glycosylation and self-proteolysis of MT1-MMP in cancer cells and that when this balance is upset the catalytically potent MT1-MMP pool is self-proteolyzed.

Original languageEnglish (US)
Pages (from-to)16897-16905
Number of pages9
JournalJournal of Biological Chemistry
Volume281
Issue number25
DOIs
StatePublished - Jun 23 2006
Externally publishedYes

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Matrix Metalloproteinase 14
Glycosylation
Autolysis
Proteolysis
N-Acetylneuraminic Acid
Hinges
Degradation
Cells
Proprotein Convertases
Furin
Dimerization
Secretory Pathway
Exocytosis
Recycling
Cell membranes
Endocytosis
Catalytic Domain
Neoplasms
Peptide Hydrolases
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

O-glycosylation regulates autolysis of cellular membrane type-1 matrix metalloproteinase (MT1-MMP). / Remacle, Albert G.; Chekanov, Alexei V.; Golubkov, Vladislav S.; Savinov, Alexei Y.; Rozanov, Dmitri; Strongin, Alex Y.

In: Journal of Biological Chemistry, Vol. 281, No. 25, 23.06.2006, p. 16897-16905.

Research output: Contribution to journalArticle

Remacle, Albert G. ; Chekanov, Alexei V. ; Golubkov, Vladislav S. ; Savinov, Alexei Y. ; Rozanov, Dmitri ; Strongin, Alex Y. / O-glycosylation regulates autolysis of cellular membrane type-1 matrix metalloproteinase (MT1-MMP). In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 25. pp. 16897-16905.
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