Nutritional Regulation of Insulin-like Growth Factor-I Receptor mRNA Levels in Growing Chickens

Yuki Matsumura, Manabu Domeki, Kunio Sugahara, Tatsuo Kubo, Charles T. Roberts, Derek Le Roith, Hisanori Kato

    Research output: Contribution to journalArticle

    29 Scopus citations

    Abstract

    Insulin-like growth factor-I (IGF-I) exerts its effect through the IGF-I receptor. To investigate the effects of nutritional status on chicken IGF-I receptor gene expression, a solution hybridization/RNase protection assay for IGF-I receptor mRNA was developed. A cDNA clone corresponding to the carboxyl- terminal region of the IGF-I receptor was obtained by reverse transcription-PCR (RT-PCR). Sequence analysis of the clone showed that this region of the chicken IGF-I receptor is highly divergent from the human IGF-I receptor. IGF-I receptor mRNA was detected in all tissues examined from newly hatched chickens. The rank order of the IGF-I receptor mRNA levels was liver < thigh muscle < stomach < heart < lung < kidney < brain. In 1-week-old chickens, 5 days of starvation caused a 2.5- to 3-fold increase in the mRNA in muscle and kidney. Starvation of 4-week-old chickens for 5 days caused a 1.7 to 2.2-fold increase in IGF-I receptor mRNA levels in kidney, liver, and muscle. In contrast, IGF-I receptor mRNA levels in brain failed to change. The mRNA levels were reduced to the control level by refeeding of the starved chickens for 24 h. These data suggest a tissue- and development-specific response of chicken IGF-I receptor gene expression to nutritional status.

    Original languageEnglish (US)
    Pages (from-to)979-982
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume60
    Issue number6
    DOIs
    StatePublished - Jan 1996

    Keywords

    • Chicken
    • Growth factor receptor
    • Insulin-like growth factor-I
    • R Nase protection assay
    • Starvation

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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