Nutritional regulation of insulin-like growth factor-I receptor mRNA levels in growing chickens

Yuki Matsumura, Manabu Domeki, Kunio Sugahara, Tatsuo Kubo, Charles Roberts, Derek LeRoith, Hisanori Kato

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    29 Scopus citations

    Abstract

    Insulin-like growth factor-I (IGF-I) exerts its effect through the IGF- I receptor. To investigate the effects of nutritional status on chicken IGF- I receptor gene expression, a solution hybridization/RNase protection assay for IGF-I receptor mRNA was developed. A cDNA clone corresponding to the carboxylterminal region of the IGF-I receptor was obtained by reverse transcription-PCR (RT-PCR). Sequence analysis of the clone showed that this region of the chicken IGF-I receptor is highly divergent from the human IGF- I receptor. IGF-I receptor mRNA was detected in all tissues examined from newly hatched chickens. The rank order of the IGF-I receptor mRNA levels was liver <thigh muscle <stomach <heart <lung <kidney <brain. In 1-week- old chickens, 5 days of starvation caused a 2.5- to 3-fold increase in the mRNA in muscle and kidney. Starvation of 4-week-old chickens for 5 days caused a 1.7 to 2.2-fold increase in IGF-I receptor mRNA levels in kidney, liver, and muscle. In contrast, IGF-I receptor mRNA levels in brain failed to change. The mRNA levels were reduced to the control level by refeeding of the starved chickens for 24 h. These data suggest a tissue- and development- specific response of chicken IGF-I receptor gene expression to nutritional status.

    Original languageEnglish (US)
    Pages (from-to)979-982
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume60
    Issue number6
    Publication statusPublished - Jun 1996

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    Keywords

    • chicken
    • growth factor receptor
    • insulin-like growth factor-I
    • RNase protection assay
    • starvation

    ASJC Scopus subject areas

    • Food Science
    • Applied Microbiology and Biotechnology
    • Chemistry (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology

    Cite this

    Matsumura, Y., Domeki, M., Sugahara, K., Kubo, T., Roberts, C., LeRoith, D., & Kato, H. (1996). Nutritional regulation of insulin-like growth factor-I receptor mRNA levels in growing chickens. Bioscience, Biotechnology and Biochemistry, 60(6), 979-982.