Nucleotide sequence of a cDNA encoding the constant region of a rabbit immunoglobulin light chain of the λ type

Robert Duvoisin, H. P. Kocher, I. Garcia, F. Rougeon, J. C. Jaton

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A cDNA library has been constructed in the plasmid pBR322 using as template 12 S poly(A)-RNA isolated from spleen cells of a hyperimmunized Basilea rabbit. One of these cDNA-containing clones was used to determine the nucleotide sequence coding for the λ light chain constant (C) region. The deduced amino acid sequence of this cDNA was found in good agreement with a Basilea rabbit C(λ) region amino acid sequence previously determined. The nucleotide sequence of the rabbit C(λ)-coding region was compared with man, mouse and chicken C(λ) sequences and showed 78%, 72% and 66% homology, respectively. Southern blot hybridization analyses of liver DNA from various rabbits were carried out. The comparison of the restriction patterns suggests that a few C(λ)-related genes occur in the rabbit genome. In addition, discrete differences in the restriction patterns may exist between rabbits of different genetic backgrounds.

Original languageEnglish (US)
Pages (from-to)379-382
Number of pages4
JournalEuropean Journal of Immunology
Volume14
Issue number4
StatePublished - 1984
Externally publishedYes

Fingerprint

Immunoglobulin Light Chains
Complementary DNA
Rabbits
Amino Acid Sequence
Southern Blotting
Gene Library
Chickens
Plasmids
Spleen
Clone Cells
Genome
Light
Messenger RNA
Liver
DNA
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Nucleotide sequence of a cDNA encoding the constant region of a rabbit immunoglobulin light chain of the λ type. / Duvoisin, Robert; Kocher, H. P.; Garcia, I.; Rougeon, F.; Jaton, J. C.

In: European Journal of Immunology, Vol. 14, No. 4, 1984, p. 379-382.

Research output: Contribution to journalArticle

@article{1d869db2a0ec4855a61786ed4f26a78e,
title = "Nucleotide sequence of a cDNA encoding the constant region of a rabbit immunoglobulin light chain of the λ type",
abstract = "A cDNA library has been constructed in the plasmid pBR322 using as template 12 S poly(A)-RNA isolated from spleen cells of a hyperimmunized Basilea rabbit. One of these cDNA-containing clones was used to determine the nucleotide sequence coding for the λ light chain constant (C) region. The deduced amino acid sequence of this cDNA was found in good agreement with a Basilea rabbit C(λ) region amino acid sequence previously determined. The nucleotide sequence of the rabbit C(λ)-coding region was compared with man, mouse and chicken C(λ) sequences and showed 78{\%}, 72{\%} and 66{\%} homology, respectively. Southern blot hybridization analyses of liver DNA from various rabbits were carried out. The comparison of the restriction patterns suggests that a few C(λ)-related genes occur in the rabbit genome. In addition, discrete differences in the restriction patterns may exist between rabbits of different genetic backgrounds.",
author = "Robert Duvoisin and Kocher, {H. P.} and I. Garcia and F. Rougeon and Jaton, {J. C.}",
year = "1984",
language = "English (US)",
volume = "14",
pages = "379--382",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Nucleotide sequence of a cDNA encoding the constant region of a rabbit immunoglobulin light chain of the λ type

AU - Duvoisin, Robert

AU - Kocher, H. P.

AU - Garcia, I.

AU - Rougeon, F.

AU - Jaton, J. C.

PY - 1984

Y1 - 1984

N2 - A cDNA library has been constructed in the plasmid pBR322 using as template 12 S poly(A)-RNA isolated from spleen cells of a hyperimmunized Basilea rabbit. One of these cDNA-containing clones was used to determine the nucleotide sequence coding for the λ light chain constant (C) region. The deduced amino acid sequence of this cDNA was found in good agreement with a Basilea rabbit C(λ) region amino acid sequence previously determined. The nucleotide sequence of the rabbit C(λ)-coding region was compared with man, mouse and chicken C(λ) sequences and showed 78%, 72% and 66% homology, respectively. Southern blot hybridization analyses of liver DNA from various rabbits were carried out. The comparison of the restriction patterns suggests that a few C(λ)-related genes occur in the rabbit genome. In addition, discrete differences in the restriction patterns may exist between rabbits of different genetic backgrounds.

AB - A cDNA library has been constructed in the plasmid pBR322 using as template 12 S poly(A)-RNA isolated from spleen cells of a hyperimmunized Basilea rabbit. One of these cDNA-containing clones was used to determine the nucleotide sequence coding for the λ light chain constant (C) region. The deduced amino acid sequence of this cDNA was found in good agreement with a Basilea rabbit C(λ) region amino acid sequence previously determined. The nucleotide sequence of the rabbit C(λ)-coding region was compared with man, mouse and chicken C(λ) sequences and showed 78%, 72% and 66% homology, respectively. Southern blot hybridization analyses of liver DNA from various rabbits were carried out. The comparison of the restriction patterns suggests that a few C(λ)-related genes occur in the rabbit genome. In addition, discrete differences in the restriction patterns may exist between rabbits of different genetic backgrounds.

UR - http://www.scopus.com/inward/record.url?scp=0021323811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021323811&partnerID=8YFLogxK

M3 - Article

C2 - 6426973

AN - SCOPUS:0021323811

VL - 14

SP - 379

EP - 382

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 4

ER -