Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene

Hiroyuki Sasaki, Koji Shimozaki, Mohamad Zubair, Nao Aoki, Kohei Ohta, Naoya Hatano, Tom Moore, Robert Feil, Miguel Constancia, Wolf Reik, Peter Rotwein

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5′ flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, we here report the complete nucleotide sequence of Igf2, including all introns and the intergenic region adjacent to Ins2. This 28-kb segment of mouse chromosome 7 exhibits 80% overall identity with the corresponding rat sequence and has a high GC content of 52%. In addition to the known CpG island within the second Igf2 promoter, another island was identified approximately 2 kb 5′ to the first exon. Other features of this locus include a 35-fold tandem repeat of an 11-bp sequence that overlaps Igf2 pseudo-exon 2, and a B2 repeat element in the intergenic region between Ins2 and Igf2. The GC-richness and the presence of CpG islands associated with tandem repeats are common features of imprinted genes and thus may play a role in the imprinting mechanism.

Original languageEnglish (US)
Pages (from-to)331-335
Number of pages5
JournalDNA Research
Volume3
Issue number5
DOIs
StatePublished - 1996

Keywords

  • CpG island
  • DNA methylation
  • GC-rich isochore
  • Genomic imprinting
  • Insulin-like growth factor II

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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