Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene

Hiroyuki Sasaki, Koji Shimozaki, Mohamad Zubair, Nao Aoki, Kohei Ohta, Naoya Hatano, Tom Moore, Robert Feil, Miguel Constancia, Wolf Reik, Peter Rotwein

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5′ flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, we here report the complete nucleotide sequence of Igf2, including all introns and the intergenic region adjacent to Ins2. This 28-kb segment of mouse chromosome 7 exhibits 80% overall identity with the corresponding rat sequence and has a high GC content of 52%. In addition to the known CpG island within the second Igf2 promoter, another island was identified approximately 2 kb 5′ to the first exon. Other features of this locus include a 35-fold tandem repeat of an 11-bp sequence that overlaps Igf2 pseudo-exon 2, and a B2 repeat element in the intergenic region between Ins2 and Igf2. The GC-richness and the presence of CpG islands associated with tandem repeats are common features of imprinted genes and thus may play a role in the imprinting mechanism.

Original languageEnglish (US)
Pages (from-to)331-335
Number of pages5
JournalDNA Research
Volume3
Issue number5
StatePublished - 1996
Externally publishedYes

Fingerprint

CpG Islands
Intergenic DNA
Tandem Repeat Sequences
Genes
Exons
Genomic Imprinting
Chromosomes, Human, Pair 7
Insulin-Like Growth Factor II
5' Flanking Region
Base Composition
Islands
Introns
Insulin

Keywords

  • CpG island
  • DNA methylation
  • GC-rich isochore
  • Genomic imprinting
  • Insulin-like growth factor II

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Sasaki, H., Shimozaki, K., Zubair, M., Aoki, N., Ohta, K., Hatano, N., ... Rotwein, P. (1996). Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene. DNA Research, 3(5), 331-335.

Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene. / Sasaki, Hiroyuki; Shimozaki, Koji; Zubair, Mohamad; Aoki, Nao; Ohta, Kohei; Hatano, Naoya; Moore, Tom; Feil, Robert; Constancia, Miguel; Reik, Wolf; Rotwein, Peter.

In: DNA Research, Vol. 3, No. 5, 1996, p. 331-335.

Research output: Contribution to journalArticle

Sasaki, H, Shimozaki, K, Zubair, M, Aoki, N, Ohta, K, Hatano, N, Moore, T, Feil, R, Constancia, M, Reik, W & Rotwein, P 1996, 'Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene', DNA Research, vol. 3, no. 5, pp. 331-335.
Sasaki H, Shimozaki K, Zubair M, Aoki N, Ohta K, Hatano N et al. Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene. DNA Research. 1996;3(5):331-335.
Sasaki, Hiroyuki ; Shimozaki, Koji ; Zubair, Mohamad ; Aoki, Nao ; Ohta, Kohei ; Hatano, Naoya ; Moore, Tom ; Feil, Robert ; Constancia, Miguel ; Reik, Wolf ; Rotwein, Peter. / Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene. In: DNA Research. 1996 ; Vol. 3, No. 5. pp. 331-335.
@article{3b98c29cfa5a4147956e1f78550a3efe,
title = "Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene",
abstract = "The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5′ flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, we here report the complete nucleotide sequence of Igf2, including all introns and the intergenic region adjacent to Ins2. This 28-kb segment of mouse chromosome 7 exhibits 80{\%} overall identity with the corresponding rat sequence and has a high GC content of 52{\%}. In addition to the known CpG island within the second Igf2 promoter, another island was identified approximately 2 kb 5′ to the first exon. Other features of this locus include a 35-fold tandem repeat of an 11-bp sequence that overlaps Igf2 pseudo-exon 2, and a B2 repeat element in the intergenic region between Ins2 and Igf2. The GC-richness and the presence of CpG islands associated with tandem repeats are common features of imprinted genes and thus may play a role in the imprinting mechanism.",
keywords = "CpG island, DNA methylation, GC-rich isochore, Genomic imprinting, Insulin-like growth factor II",
author = "Hiroyuki Sasaki and Koji Shimozaki and Mohamad Zubair and Nao Aoki and Kohei Ohta and Naoya Hatano and Tom Moore and Robert Feil and Miguel Constancia and Wolf Reik and Peter Rotwein",
year = "1996",
language = "English (US)",
volume = "3",
pages = "331--335",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene

AU - Sasaki, Hiroyuki

AU - Shimozaki, Koji

AU - Zubair, Mohamad

AU - Aoki, Nao

AU - Ohta, Kohei

AU - Hatano, Naoya

AU - Moore, Tom

AU - Feil, Robert

AU - Constancia, Miguel

AU - Reik, Wolf

AU - Rotwein, Peter

PY - 1996

Y1 - 1996

N2 - The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5′ flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, we here report the complete nucleotide sequence of Igf2, including all introns and the intergenic region adjacent to Ins2. This 28-kb segment of mouse chromosome 7 exhibits 80% overall identity with the corresponding rat sequence and has a high GC content of 52%. In addition to the known CpG island within the second Igf2 promoter, another island was identified approximately 2 kb 5′ to the first exon. Other features of this locus include a 35-fold tandem repeat of an 11-bp sequence that overlaps Igf2 pseudo-exon 2, and a B2 repeat element in the intergenic region between Ins2 and Igf2. The GC-richness and the presence of CpG islands associated with tandem repeats are common features of imprinted genes and thus may play a role in the imprinting mechanism.

AB - The mouse insulin-like growth factor II gene (Igf2) is physically linked to the insulin II gene (Ins2) and both are subject to tissue-specific genomic imprinting. The paternal-specific expression of Igf2 has been associated with hypermethylation of some CpG sites in the 5′ flanking region and in the body of the gene. As a first step in analyzing the structural features of this imprinted locus, we here report the complete nucleotide sequence of Igf2, including all introns and the intergenic region adjacent to Ins2. This 28-kb segment of mouse chromosome 7 exhibits 80% overall identity with the corresponding rat sequence and has a high GC content of 52%. In addition to the known CpG island within the second Igf2 promoter, another island was identified approximately 2 kb 5′ to the first exon. Other features of this locus include a 35-fold tandem repeat of an 11-bp sequence that overlaps Igf2 pseudo-exon 2, and a B2 repeat element in the intergenic region between Ins2 and Igf2. The GC-richness and the presence of CpG islands associated with tandem repeats are common features of imprinted genes and thus may play a role in the imprinting mechanism.

KW - CpG island

KW - DNA methylation

KW - GC-rich isochore

KW - Genomic imprinting

KW - Insulin-like growth factor II

UR - http://www.scopus.com/inward/record.url?scp=0030608276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030608276&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 331

EP - 335

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 5

ER -