Nucleotide sequence of 5′ portion of srfA that contains the region required for competence establishment in Bacillus subtilus

Shoichi Fuma, Yoshiyuki Fujishima, Nathan Corbell, Cletus D'Souza, Michiko Nakano, Peter Zuber, Kunio Yamane

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region, the first open reading frame, srfAA, encoding surfactin synthetase I and part of the second open reading frame, srfAB, encoding surfactin synthetase II. Three amino acid-activating domains characteristic of those found in peptide synthetases could be discerned in both srfAA (activating Glu, Leu and D-Leu) and srfAB (activating Val, Asp, and D-Leu). The presence of a conserved spacer motif in the amino-terminal end of srfAA suggests that the srfAA product may not initiate surfactin synthesis. The portion of srfA that contains the region required for competence is composed of srfAA and the first amino acid-acitivating domain of srfAB.

Original languageEnglish (US)
Pages (from-to)93-97
Number of pages5
JournalNucleic Acids Research
Volume21
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

Bacilli
Nucleotides
Mental Competency
Bacillus
Open Reading Frames
Amino acids
Peptide Synthases
Amino Acids
Encoding
Operon
Viperidae
Genetic Promoter Regions
Base Pairing
Peptides
Promoter
Synthesis
surfactin synthetase
Nucleotide sequence

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Nucleotide sequence of 5′ portion of srfA that contains the region required for competence establishment in Bacillus subtilus. / Fuma, Shoichi; Fujishima, Yoshiyuki; Corbell, Nathan; D'Souza, Cletus; Nakano, Michiko; Zuber, Peter; Yamane, Kunio.

In: Nucleic Acids Research, Vol. 21, No. 1, 1993, p. 93-97.

Research output: Contribution to journalArticle

Fuma, Shoichi ; Fujishima, Yoshiyuki ; Corbell, Nathan ; D'Souza, Cletus ; Nakano, Michiko ; Zuber, Peter ; Yamane, Kunio. / Nucleotide sequence of 5′ portion of srfA that contains the region required for competence establishment in Bacillus subtilus. In: Nucleic Acids Research. 1993 ; Vol. 21, No. 1. pp. 93-97.
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AU - Nakano, Michiko

AU - Zuber, Peter

AU - Yamane, Kunio

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