TY - JOUR
T1 - Nucleotide sequence of 5' portion of srfA that contains the region required for competence establishment in Bacillus subtilus
AU - Fuma, Shoichi
AU - Fujishima, Yoshiyuki
AU - Corbell, Nathan
AU - D'souza, Cletus
AU - Nakano, Michiko M.
AU - Zuber, Peter
AU - Yamane, Kunio
N1 - Funding Information:
The authors are grateful to M.Itaya for Not I linking clones, N.Ogasawara for X dash library, J.Vater, D.Vollenbreich, and M.A.Marahiel for information and valuable discussion. This research was supported by a grant-in-aid from Ministry of Education, Sciences, and Culture of Japan (to K. Y.); and grants GM39476 (to P.Z.) and GM45898 (to P.Z.) from the National Institutes of Health, USA. The GenBank/EMBL/DDBJ accession number for the seqeunce presented is D13262.
PY - 1993/1/11
Y1 - 1993/1/11
N2 - The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region, the first open reading frame, srfAA, encoding surfactin synthetase I and part of the second open reading frame, srfAB, encoding surfactin synthetase II. Three amino acid-activating domains characteristic of those found in peptide synthetases could be discerned In both srfAA (activating Glu, Leu and D-Leu) and srfAB (activating Val, Asp, and D-Leu). The presence of a conserved spacer motif in the amino-terminal end of srfAA suggests that the srfAA product may not initiate surfactin synthesis. The portion of srfA that contains the region required for competence is composed of srfAA and the first amino acid-acltivating domain of srfAB.
AB - The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region, the first open reading frame, srfAA, encoding surfactin synthetase I and part of the second open reading frame, srfAB, encoding surfactin synthetase II. Three amino acid-activating domains characteristic of those found in peptide synthetases could be discerned In both srfAA (activating Glu, Leu and D-Leu) and srfAB (activating Val, Asp, and D-Leu). The presence of a conserved spacer motif in the amino-terminal end of srfAA suggests that the srfAA product may not initiate surfactin synthesis. The portion of srfA that contains the region required for competence is composed of srfAA and the first amino acid-acltivating domain of srfAB.
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U2 - 10.1093/nar/21.1.93
DO - 10.1093/nar/21.1.93
M3 - Article
C2 - 8441623
AN - SCOPUS:0027299621
SN - 0305-1048
VL - 21
SP - 93
EP - 97
JO - Nucleic acids research
JF - Nucleic acids research
IS - 1
ER -