Nucleotide binding of the C-terminal domains of the major histocompatibility complex-encoded transporter expressed in Drosophila melanogaster cells

Kena Wang, Klaus Früh, Per A. Peterson, Young Yang

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The C-terminal domains of the mouse transporter associated with antigen processing (TAP) were expressed as soluble proteins in Drosophila melanogaster cells and labeled by [α-32P]8-azido-ATP after UV-irradiation. The relative potencies of the nucleotides in preventing azido-ATP labeling were in the order of ATP > GTP > CTP > ITP > UTP for both the TAP1 and TAP2 C-terminal domains, suggesting ATP to be the natural substrate of the transporter. Our data provide the first evidence that the individual C-terminal domain of either TAP1 or TAP2 can be expressed as a functional ATP-binding protein.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalFEBS Letters
Volume350
Issue number2-3
DOIs
StatePublished - Aug 22 1994

Keywords

  • ATP-binding cassette transporter
  • Antigen presentation
  • Major histocompatibility complex
  • Traffic ATP-ase
  • Transporter associated with antigen processing

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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