Nucleolar organizer regions in iris nevi and melanomas

D. M. Marcus, L. A. Mawn, K. M. Egan, Daniel Albert

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The silver staining of nucleolar organizer region-associated proteins is an objective method that has been used to differentiate benign from malignant neoplasms. Recently this method was used to distinguish benign choroidal nevi from malignant choroidal melanomas. We studied 24 iris melanocytic lesions to assess the applicability of this technique for differentiating benign from neoplastic iris tumors. Masked observers determined the number of silver- stained nucleolar organizer region dots per cell for silver-stained specimens. Iris nevi contained a mean of 1.6 silver-stained nucleolar organizer region dots per cell, whereas iris (spindle A and B, spindle B, epithelioid, mixed cell) malignant melanomas contained a mean of at least 3.5 silver-stained nucleolar organizer region dots per cell (P < .0001). All iris nevi demonstrated counts lower than 1.9, whereas all iris melanomas demonstrated counts greater than 2.8. Silver-stained nucleolar organizer region counts were also compared with the clinico-pathologic variables of gender, age, and largest specimen dimension. Only the largest specimen dimension correlated with silver-stained nucleolar organizer region counts (P < .0029). The silver-stained nucleolar organizer region method is a simple technique for differentiating iris nevi from iris melanomas. The silver- stained nucleolar organizer region technique may aid in the assessment and treatment of iris lesions by confirming the malignancy of biopsy specimens.

Original languageEnglish (US)
Pages (from-to)202-207
Number of pages6
JournalAmerican Journal of Ophthalmology
Volume114
Issue number2
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Nevi and Melanomas
Nucleolus Organizer Region
Iris
Silver
Nevus
Melanoma
Epithelioid Cells
Neoplasms
Silver Staining
Biopsy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Nucleolar organizer regions in iris nevi and melanomas. / Marcus, D. M.; Mawn, L. A.; Egan, K. M.; Albert, Daniel.

In: American Journal of Ophthalmology, Vol. 114, No. 2, 01.01.1992, p. 202-207.

Research output: Contribution to journalArticle

Marcus, D. M. ; Mawn, L. A. ; Egan, K. M. ; Albert, Daniel. / Nucleolar organizer regions in iris nevi and melanomas. In: American Journal of Ophthalmology. 1992 ; Vol. 114, No. 2. pp. 202-207.
@article{3334dcf769a84ea09a3366abf595c9c0,
title = "Nucleolar organizer regions in iris nevi and melanomas",
abstract = "The silver staining of nucleolar organizer region-associated proteins is an objective method that has been used to differentiate benign from malignant neoplasms. Recently this method was used to distinguish benign choroidal nevi from malignant choroidal melanomas. We studied 24 iris melanocytic lesions to assess the applicability of this technique for differentiating benign from neoplastic iris tumors. Masked observers determined the number of silver- stained nucleolar organizer region dots per cell for silver-stained specimens. Iris nevi contained a mean of 1.6 silver-stained nucleolar organizer region dots per cell, whereas iris (spindle A and B, spindle B, epithelioid, mixed cell) malignant melanomas contained a mean of at least 3.5 silver-stained nucleolar organizer region dots per cell (P < .0001). All iris nevi demonstrated counts lower than 1.9, whereas all iris melanomas demonstrated counts greater than 2.8. Silver-stained nucleolar organizer region counts were also compared with the clinico-pathologic variables of gender, age, and largest specimen dimension. Only the largest specimen dimension correlated with silver-stained nucleolar organizer region counts (P < .0029). The silver-stained nucleolar organizer region method is a simple technique for differentiating iris nevi from iris melanomas. The silver- stained nucleolar organizer region technique may aid in the assessment and treatment of iris lesions by confirming the malignancy of biopsy specimens.",
author = "Marcus, {D. M.} and Mawn, {L. A.} and Egan, {K. M.} and Daniel Albert",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/S0002-9394(14)73985-6",
language = "English (US)",
volume = "114",
pages = "202--207",
journal = "American Journal of Ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - Nucleolar organizer regions in iris nevi and melanomas

AU - Marcus, D. M.

AU - Mawn, L. A.

AU - Egan, K. M.

AU - Albert, Daniel

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The silver staining of nucleolar organizer region-associated proteins is an objective method that has been used to differentiate benign from malignant neoplasms. Recently this method was used to distinguish benign choroidal nevi from malignant choroidal melanomas. We studied 24 iris melanocytic lesions to assess the applicability of this technique for differentiating benign from neoplastic iris tumors. Masked observers determined the number of silver- stained nucleolar organizer region dots per cell for silver-stained specimens. Iris nevi contained a mean of 1.6 silver-stained nucleolar organizer region dots per cell, whereas iris (spindle A and B, spindle B, epithelioid, mixed cell) malignant melanomas contained a mean of at least 3.5 silver-stained nucleolar organizer region dots per cell (P < .0001). All iris nevi demonstrated counts lower than 1.9, whereas all iris melanomas demonstrated counts greater than 2.8. Silver-stained nucleolar organizer region counts were also compared with the clinico-pathologic variables of gender, age, and largest specimen dimension. Only the largest specimen dimension correlated with silver-stained nucleolar organizer region counts (P < .0029). The silver-stained nucleolar organizer region method is a simple technique for differentiating iris nevi from iris melanomas. The silver- stained nucleolar organizer region technique may aid in the assessment and treatment of iris lesions by confirming the malignancy of biopsy specimens.

AB - The silver staining of nucleolar organizer region-associated proteins is an objective method that has been used to differentiate benign from malignant neoplasms. Recently this method was used to distinguish benign choroidal nevi from malignant choroidal melanomas. We studied 24 iris melanocytic lesions to assess the applicability of this technique for differentiating benign from neoplastic iris tumors. Masked observers determined the number of silver- stained nucleolar organizer region dots per cell for silver-stained specimens. Iris nevi contained a mean of 1.6 silver-stained nucleolar organizer region dots per cell, whereas iris (spindle A and B, spindle B, epithelioid, mixed cell) malignant melanomas contained a mean of at least 3.5 silver-stained nucleolar organizer region dots per cell (P < .0001). All iris nevi demonstrated counts lower than 1.9, whereas all iris melanomas demonstrated counts greater than 2.8. Silver-stained nucleolar organizer region counts were also compared with the clinico-pathologic variables of gender, age, and largest specimen dimension. Only the largest specimen dimension correlated with silver-stained nucleolar organizer region counts (P < .0029). The silver-stained nucleolar organizer region method is a simple technique for differentiating iris nevi from iris melanomas. The silver- stained nucleolar organizer region technique may aid in the assessment and treatment of iris lesions by confirming the malignancy of biopsy specimens.

UR - http://www.scopus.com/inward/record.url?scp=0026670022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026670022&partnerID=8YFLogxK

U2 - 10.1016/S0002-9394(14)73985-6

DO - 10.1016/S0002-9394(14)73985-6

M3 - Article

C2 - 1642296

AN - SCOPUS:0026670022

VL - 114

SP - 202

EP - 207

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

SN - 0002-9394

IS - 2

ER -