Nuclear magnetic resonance assessment of adenosine triphosphate (Atp) dynamics in ischemic mouse livers perfused with adenine and ribose

Robert G. Lee, Charles Jr Springer, Paul Kasulis, Amos Lanir, Jonathan Frazer, Melvin E. Clouse

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Hepatic energy stores are essential to liver viability. We used a mouse liver perfusion model and MR spectroscopy to study the effect of adding two precursors of ATP (adenine and ribose) on ATP dynamics during ischemia and reperfusion. Using Krebs-Henseleit buffer with or without added adenine and ribose made little difference in the ATP decay rate during ischemia, but the recovery of ATP during reperfusion was more complete when adenine and ribose were added to the buffer. These findings suggest that the addition of the precursors of ATP, adenine and ribose, to perfusate after ischemia can accelerate and enhance ATP recovery.

Original languageEnglish (US)
Pages (from-to)685-687
Number of pages3
JournalInvestigative Radiology
Volume22
Issue number8
StatePublished - 1987
Externally publishedYes

Fingerprint

Ribose
Adenine
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Liver
Ischemia
Reperfusion
Buffers
Perfusion

Keywords

  • ATP
  • Ischemia
  • Liver
  • NMR

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Nuclear magnetic resonance assessment of adenosine triphosphate (Atp) dynamics in ischemic mouse livers perfused with adenine and ribose. / Lee, Robert G.; Springer, Charles Jr; Kasulis, Paul; Lanir, Amos; Frazer, Jonathan; Clouse, Melvin E.

In: Investigative Radiology, Vol. 22, No. 8, 1987, p. 685-687.

Research output: Contribution to journalArticle

Lee, Robert G. ; Springer, Charles Jr ; Kasulis, Paul ; Lanir, Amos ; Frazer, Jonathan ; Clouse, Melvin E. / Nuclear magnetic resonance assessment of adenosine triphosphate (Atp) dynamics in ischemic mouse livers perfused with adenine and ribose. In: Investigative Radiology. 1987 ; Vol. 22, No. 8. pp. 685-687.
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AU - Frazer, Jonathan

AU - Clouse, Melvin E.

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