Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling

Sean Speese, James Ashley, Vahbiz Jokhi, John Nunnari, Romina Barria, Yihang Li, Bulent Ataman, Alex Koon, Young Tae Chang, Qian Li, Melissa J. Moore, Vivian Budnik

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

Localized protein synthesis requires assembly and transport of translationally silenced ribonucleoprotein particles (RNPs), some of which are exceptionally large. Where in the cell such large RNP granules first assemble was heretofore unknown. We previously reported that during synapse development, a fragment of the Wnt-1 receptor, DFrizzled2, enters postsynaptic nuclei where it forms prominent foci. Here we show that these foci constitute large RNP granules harboring synaptic protein transcripts. These granules exit the nucleus by budding through the inner and the outer nuclear membranes in a nuclear egress mechanism akin to that of herpes viruses. This budding involves phosphorylation of A-type lamin, a protein linked to muscular dystrophies. Thus nuclear envelope budding is an endogenous nuclear export pathway for large RNP granules. PaperFlick:

Original languageEnglish (US)
Pages (from-to)832-846
Number of pages15
JournalCell
Volume149
Issue number4
DOIs
StatePublished - May 11 2012

Fingerprint

Ribonucleoproteins
Nuclear Envelope
Wnt Receptors
Lamin Type A
Phosphorylation
Proteins
Cell Nucleus Active Transport
Muscular Dystrophies
Viruses
Synapses
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling. / Speese, Sean; Ashley, James; Jokhi, Vahbiz; Nunnari, John; Barria, Romina; Li, Yihang; Ataman, Bulent; Koon, Alex; Chang, Young Tae; Li, Qian; Moore, Melissa J.; Budnik, Vivian.

In: Cell, Vol. 149, No. 4, 11.05.2012, p. 832-846.

Research output: Contribution to journalArticle

Speese, S, Ashley, J, Jokhi, V, Nunnari, J, Barria, R, Li, Y, Ataman, B, Koon, A, Chang, YT, Li, Q, Moore, MJ & Budnik, V 2012, 'Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling', Cell, vol. 149, no. 4, pp. 832-846. https://doi.org/10.1016/j.cell.2012.03.032
Speese, Sean ; Ashley, James ; Jokhi, Vahbiz ; Nunnari, John ; Barria, Romina ; Li, Yihang ; Ataman, Bulent ; Koon, Alex ; Chang, Young Tae ; Li, Qian ; Moore, Melissa J. ; Budnik, Vivian. / Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling. In: Cell. 2012 ; Vol. 149, No. 4. pp. 832-846.
@article{7a9600c7b2094f11a10950bb6f83c224,
title = "Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling",
abstract = "Localized protein synthesis requires assembly and transport of translationally silenced ribonucleoprotein particles (RNPs), some of which are exceptionally large. Where in the cell such large RNP granules first assemble was heretofore unknown. We previously reported that during synapse development, a fragment of the Wnt-1 receptor, DFrizzled2, enters postsynaptic nuclei where it forms prominent foci. Here we show that these foci constitute large RNP granules harboring synaptic protein transcripts. These granules exit the nucleus by budding through the inner and the outer nuclear membranes in a nuclear egress mechanism akin to that of herpes viruses. This budding involves phosphorylation of A-type lamin, a protein linked to muscular dystrophies. Thus nuclear envelope budding is an endogenous nuclear export pathway for large RNP granules. PaperFlick:",
author = "Sean Speese and James Ashley and Vahbiz Jokhi and John Nunnari and Romina Barria and Yihang Li and Bulent Ataman and Alex Koon and Chang, {Young Tae} and Qian Li and Moore, {Melissa J.} and Vivian Budnik",
year = "2012",
month = "5",
day = "11",
doi = "10.1016/j.cell.2012.03.032",
language = "English (US)",
volume = "149",
pages = "832--846",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling

AU - Speese, Sean

AU - Ashley, James

AU - Jokhi, Vahbiz

AU - Nunnari, John

AU - Barria, Romina

AU - Li, Yihang

AU - Ataman, Bulent

AU - Koon, Alex

AU - Chang, Young Tae

AU - Li, Qian

AU - Moore, Melissa J.

AU - Budnik, Vivian

PY - 2012/5/11

Y1 - 2012/5/11

N2 - Localized protein synthesis requires assembly and transport of translationally silenced ribonucleoprotein particles (RNPs), some of which are exceptionally large. Where in the cell such large RNP granules first assemble was heretofore unknown. We previously reported that during synapse development, a fragment of the Wnt-1 receptor, DFrizzled2, enters postsynaptic nuclei where it forms prominent foci. Here we show that these foci constitute large RNP granules harboring synaptic protein transcripts. These granules exit the nucleus by budding through the inner and the outer nuclear membranes in a nuclear egress mechanism akin to that of herpes viruses. This budding involves phosphorylation of A-type lamin, a protein linked to muscular dystrophies. Thus nuclear envelope budding is an endogenous nuclear export pathway for large RNP granules. PaperFlick:

AB - Localized protein synthesis requires assembly and transport of translationally silenced ribonucleoprotein particles (RNPs), some of which are exceptionally large. Where in the cell such large RNP granules first assemble was heretofore unknown. We previously reported that during synapse development, a fragment of the Wnt-1 receptor, DFrizzled2, enters postsynaptic nuclei where it forms prominent foci. Here we show that these foci constitute large RNP granules harboring synaptic protein transcripts. These granules exit the nucleus by budding through the inner and the outer nuclear membranes in a nuclear egress mechanism akin to that of herpes viruses. This budding involves phosphorylation of A-type lamin, a protein linked to muscular dystrophies. Thus nuclear envelope budding is an endogenous nuclear export pathway for large RNP granules. PaperFlick:

UR - http://www.scopus.com/inward/record.url?scp=84860852545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860852545&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2012.03.032

DO - 10.1016/j.cell.2012.03.032

M3 - Article

C2 - 22579286

AN - SCOPUS:84860852545

VL - 149

SP - 832

EP - 846

JO - Cell

JF - Cell

SN - 0092-8674

IS - 4

ER -