@article{7a9600c7b2094f11a10950bb6f83c224,
title = "Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic Wnt signaling",
abstract = "Localized protein synthesis requires assembly and transport of translationally silenced ribonucleoprotein particles (RNPs), some of which are exceptionally large. Where in the cell such large RNP granules first assemble was heretofore unknown. We previously reported that during synapse development, a fragment of the Wnt-1 receptor, DFrizzled2, enters postsynaptic nuclei where it forms prominent foci. Here we show that these foci constitute large RNP granules harboring synaptic protein transcripts. These granules exit the nucleus by budding through the inner and the outer nuclear membranes in a nuclear egress mechanism akin to that of herpes viruses. This budding involves phosphorylation of A-type lamin, a protein linked to muscular dystrophies. Thus nuclear envelope budding is an endogenous nuclear export pathway for large RNP granules. PaperFlick:",
author = "Speese, {Sean D.} and James Ashley and Vahbiz Jokhi and John Nunnari and Romina Barria and Yihang Li and Bulent Ataman and Alex Koon and Chang, {Young Tae} and Qian Li and Moore, {Melissa J.} and Vivian Budnik",
note = "Funding Information: We thank Drs. Thoru Pederson and Jeanne Lawrence for helpful advice and discussions. We also thank Dr. Kate Koles and Ms. Yuly Fuentes-Medel for support in some of the molecular studies, as well as Stefanie Kaech Petrie and Aurelie Snyder of the Jungers Center Advance Microscopy Core for help with structured illumination images. Finally, we are grateful to the UMassMed Electron Microscopy Facility for support on ultrastructural studies, and Aaron DiAntonio, Martine Simonelig, and Lori Wallrath for reagent sharing. This work was supported by an NRSA to S.D.S., NIH grant RO1NS063228 to V.B., and core resources from DERC grant DK32520. M.J.M. is a HHMI investigator. S.D.S. designed and conducted many of the experiments and contributed intellectually to the project and manuscript writing. J.A. conducted FISH, live imaging, and contributed intellectually to the project and manuscript editing. J.N. conducted EM. R.B. and Y.L. provided technical support. V.J. performed experiments related to aPKC, and contributed to experimental design. B.A. was the first to discover that nuclear DFz2C foci where localized at LamC foci, and that synaptic activity regulated the number of LamC foci in conjunction with DFz2C foci. A.K. contributed to live imaging. Y.-T.C. and Q.L. supplied the E36 dye. M.J.M. provided intellectual input, contributed to experimental design, and collaborated in writing the manuscript. V.B. directed the project and contributed to experimental design, discussions, and manuscript writing. ",
year = "2012",
month = may,
day = "11",
doi = "10.1016/j.cell.2012.03.032",
language = "English (US)",
volume = "149",
pages = "832--846",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}