Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins

Martina Maric, Alison C. Haugo, William Dauer, David Johnson, Richard J. Roller

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Herpesvirus infection reorganizes components of the nuclear lamina usually without loss of integrity of the nuclear membranes. We report that wild-type HSV infection can cause dissolution of the nuclear envelope in transformed mouse embryonic fibroblasts that do not express torsinA. Nuclear envelope breakdown is accompanied by an eight-fold inhibition of virus replication. Breakdown of the membrane is much more limited during infection with viruses that lack the gB and gH genes, suggesting that breakdown involves factors that promote fusion at the nuclear membrane. Nuclear envelope breakdown is also inhibited during infection with virus that does not express UL34, but is enhanced when the US3 gene is deleted, suggesting that envelope breakdown may be enhanced by nuclear lamina disruption. Nuclear envelope breakdown cannot compensate for deletion of the UL34 gene suggesting that mixing of nuclear and cytoplasmic contents is insufficient to bypass loss of the normal nuclear egress pathway.

Original languageEnglish (US)
Pages (from-to)128-137
Number of pages10
JournalVirology
Volume460-461
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Viral Fusion Proteins
Nuclear Envelope
Human Herpesvirus 1
Nuclear Lamina
Virus Diseases
Herpesviridae Infections
Gene Deletion
Virus Replication
Genes
Fibroblasts
Membranes
Infection

Keywords

  • Fusion
  • GB
  • GH
  • HSV-1
  • Nuclear envelope
  • UL34
  • US3

ASJC Scopus subject areas

  • Virology

Cite this

Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins. / Maric, Martina; Haugo, Alison C.; Dauer, William; Johnson, David; Roller, Richard J.

In: Virology, Vol. 460-461, No. 1, 2014, p. 128-137.

Research output: Contribution to journalArticle

Maric, Martina ; Haugo, Alison C. ; Dauer, William ; Johnson, David ; Roller, Richard J. / Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins. In: Virology. 2014 ; Vol. 460-461, No. 1. pp. 128-137.
@article{4c73fd3619864f8aaed2ab0a0b126a62,
title = "Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins",
abstract = "Herpesvirus infection reorganizes components of the nuclear lamina usually without loss of integrity of the nuclear membranes. We report that wild-type HSV infection can cause dissolution of the nuclear envelope in transformed mouse embryonic fibroblasts that do not express torsinA. Nuclear envelope breakdown is accompanied by an eight-fold inhibition of virus replication. Breakdown of the membrane is much more limited during infection with viruses that lack the gB and gH genes, suggesting that breakdown involves factors that promote fusion at the nuclear membrane. Nuclear envelope breakdown is also inhibited during infection with virus that does not express UL34, but is enhanced when the US3 gene is deleted, suggesting that envelope breakdown may be enhanced by nuclear lamina disruption. Nuclear envelope breakdown cannot compensate for deletion of the UL34 gene suggesting that mixing of nuclear and cytoplasmic contents is insufficient to bypass loss of the normal nuclear egress pathway.",
keywords = "Fusion, GB, GH, HSV-1, Nuclear envelope, UL34, US3",
author = "Martina Maric and Haugo, {Alison C.} and William Dauer and David Johnson and Roller, {Richard J.}",
year = "2014",
doi = "10.1016/j.virol.2014.05.010",
language = "English (US)",
volume = "460-461",
pages = "128--137",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Nuclear envelope breakdown induced by herpes simplex virus type 1 involves the activity of viral fusion proteins

AU - Maric, Martina

AU - Haugo, Alison C.

AU - Dauer, William

AU - Johnson, David

AU - Roller, Richard J.

PY - 2014

Y1 - 2014

N2 - Herpesvirus infection reorganizes components of the nuclear lamina usually without loss of integrity of the nuclear membranes. We report that wild-type HSV infection can cause dissolution of the nuclear envelope in transformed mouse embryonic fibroblasts that do not express torsinA. Nuclear envelope breakdown is accompanied by an eight-fold inhibition of virus replication. Breakdown of the membrane is much more limited during infection with viruses that lack the gB and gH genes, suggesting that breakdown involves factors that promote fusion at the nuclear membrane. Nuclear envelope breakdown is also inhibited during infection with virus that does not express UL34, but is enhanced when the US3 gene is deleted, suggesting that envelope breakdown may be enhanced by nuclear lamina disruption. Nuclear envelope breakdown cannot compensate for deletion of the UL34 gene suggesting that mixing of nuclear and cytoplasmic contents is insufficient to bypass loss of the normal nuclear egress pathway.

AB - Herpesvirus infection reorganizes components of the nuclear lamina usually without loss of integrity of the nuclear membranes. We report that wild-type HSV infection can cause dissolution of the nuclear envelope in transformed mouse embryonic fibroblasts that do not express torsinA. Nuclear envelope breakdown is accompanied by an eight-fold inhibition of virus replication. Breakdown of the membrane is much more limited during infection with viruses that lack the gB and gH genes, suggesting that breakdown involves factors that promote fusion at the nuclear membrane. Nuclear envelope breakdown is also inhibited during infection with virus that does not express UL34, but is enhanced when the US3 gene is deleted, suggesting that envelope breakdown may be enhanced by nuclear lamina disruption. Nuclear envelope breakdown cannot compensate for deletion of the UL34 gene suggesting that mixing of nuclear and cytoplasmic contents is insufficient to bypass loss of the normal nuclear egress pathway.

KW - Fusion

KW - GB

KW - GH

KW - HSV-1

KW - Nuclear envelope

KW - UL34

KW - US3

UR - http://www.scopus.com/inward/record.url?scp=84901507034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901507034&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2014.05.010

DO - 10.1016/j.virol.2014.05.010

M3 - Article

VL - 460-461

SP - 128

EP - 137

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -