Nuclear entry, oligomerization, and DNA binding of the Drosophila heat shock transcription factor are regulated by a unique nuclear localization sequence

Ebrahim Zandi, Thanh Nga T Tran, Winston Chamberlain, Carl S. Parker

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In normally growing Drosophila cultured cells the Drosophila heat shock transcription factor (dHSF) is localized in the cytosol and translocates into the nucleus after heat shock. In the cytosol of nonshocked cells, the dHSF is present as a monomer that cannot bind DNA. Upon stress, the dHSF enters the nucleus where it is observed to be a trimer. A novel nuclear localization sequence (NLS) in the dHSF was found to be responsible for stress-dependent nuclear entry. Deletion of the NLS prevents nuclear entry, as expected, yet surprisingly also allows constitutive oligomerization and DNA binding in the cytosol. Further analysis of the NLS by mutagenesis suggests that the two functions of nuclear entry and oligomerization are separable in that distinct residues present in the NLS are responsible for each. Mutations in certain basic residues completely block nuclear entry, as expected for a constitutive NLS. In addition, two residues were found in the NLS that, when altered, allowed constitutive nuclear entry of dHSF independent of stress. These residues may interact with a putative cellular component or possibly other domains of the HSF to prevent nuclear entry in normally growing cells. The NLS can also function autonomously to target a β-galactosidase fusion protein into the nucleus in a heat shock-dependent fashion.

Original languageEnglish (US)
Pages (from-to)1299-1314
Number of pages16
JournalGenes and Development
Volume11
Issue number10
StatePublished - May 15 1997
Externally publishedYes

Fingerprint

Cytosol
DNA
Shock
Hot Temperature
Galactosidases
Mutagenesis
Drosophila
Cultured Cells
Drosophila Hsf protein
Mutation
Proteins

Keywords

  • DNA-binding regulation
  • nuclear localization
  • Transcription regulation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Nuclear entry, oligomerization, and DNA binding of the Drosophila heat shock transcription factor are regulated by a unique nuclear localization sequence. / Zandi, Ebrahim; Tran, Thanh Nga T; Chamberlain, Winston; Parker, Carl S.

In: Genes and Development, Vol. 11, No. 10, 15.05.1997, p. 1299-1314.

Research output: Contribution to journalArticle

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