Novel surface markers directed against adult human gallbladder

Feorillo H. Galivo, Craig S. Dorrell, Maria Grompe, Yong Ping Zhong, Philip Streeter, Markus Grompe

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Novel cell surface-reactive monoclonal antibodies generated against extrahepatic biliary cells were developed for the isolation and characterization of different cell subsets from normal adult human gallbladder. Eleven antigenically distinct gallbladder subpopulations were isolated by fluorescence-activated cell sorting. They were classified into epithelial, mesenchymal, and pancreatobiliary (PDX1+SOX9+) subsets based on gene expression profiling. These antigenically distinct human gallbladder cell subsets could potentially also reflect different functional properties in regards to bile physiology, cell renewal and plasticity. Three of the novel monoclonal antibodies differentially labeled archival sections of primary carcinoma of human gallbladder relative to normal tissue. The novel monoclonal antibodies described herein enable the identification and characterization of antigenically diverse cell subsets within adult human gallbladder and are putative tumor biomarkers.

Original languageEnglish (US)
Pages (from-to)172-181
Number of pages10
JournalStem Cell Research
Volume15
Issue number1
DOIs
StatePublished - Jul 1 2015

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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    Galivo, F. H., Dorrell, C. S., Grompe, M., Zhong, Y. P., Streeter, P., & Grompe, M. (2015). Novel surface markers directed against adult human gallbladder. Stem Cell Research, 15(1), 172-181. https://doi.org/10.1016/j.scr.2015.06.004