TY - JOUR
T1 - Novel primate miRNAs coevolved with ancient target genes in germinal zone-specific expression patterns
AU - Arcila, Mary L.
AU - Betizeau, Marion
AU - Cambronne, Xiaolu A.
AU - Guzman, Elmer
AU - Doerflinger, Nathalie
AU - Bouhallier, Frantz
AU - Zhou, Hongjun
AU - Wu, Bian
AU - Rani, Neha
AU - Bassett, Danielle S.
AU - Borello, Ugo
AU - Huissoud, Cyril
AU - Goodman, Richard H.
AU - Dehay, Colette
AU - Kosik, Kenneth S.
N1 - Funding Information:
We thank Henry Kennedy for critically reading the manuscript, V. Cortay, C. Manesse, and A. Bellemin-Ménard for technical assistance, and B. Beneyton, M. Valdebenito, and M. Séon for excellent animal care. This work was supported by the Sheldon and Miriam Adelson Medical Foundation, LABEX CORTEX (ANR-11-LABX-0042), LABEX DEVWECAN (ANR-10-LABX-0061) of Université de Lyon (ANR-11-IDEX-0007) operated by the French National Research Agency (ANR), Fondation Recherche Médicale (ING20130526653) and Neurodis young investigator award to U.B. X.A.C. was involved in the commercial licensing of the RISC-trap technology. This did not influence the experimental design, data collection, or analysis of the project data.
PY - 2014/3/19
Y1 - 2014/3/19
N2 - Major nonprimate-primate differences in corticogenesis include the dimensions, precursor lineages, and developmental timing of the germinal zones (GZs). microRNAs (miRNAs) of laser-dissected GZ compartments and cortical plate (CP) from embryonic E80 macaque visual cortex were deep sequenced. The CP and the GZ including ventricular zone (VZ) and outer and inner subcompartments of the outer subventricular zone (OSVZ) in area 17 displayed unique miRNA profiles. miRNAs present in primate, but absent in rodent, contributed disproportionately to the differential expression between GZ subregions. Prominent among the validated targets of these miRNAs were cell-cycle and neurogenesis regulators. Coevolution between the emergent miRNAs and their targets suggested that novel miRNAs became integrated into ancient gene circuitry to exert additional control over proliferation. We conclude that multiple cell-cycle regulatory events contribute to the emergence of primate-specific cortical features, including the OSVZ, generated enlarged supragranular layers, largely responsible for the increased primate cortex computational abilities.
AB - Major nonprimate-primate differences in corticogenesis include the dimensions, precursor lineages, and developmental timing of the germinal zones (GZs). microRNAs (miRNAs) of laser-dissected GZ compartments and cortical plate (CP) from embryonic E80 macaque visual cortex were deep sequenced. The CP and the GZ including ventricular zone (VZ) and outer and inner subcompartments of the outer subventricular zone (OSVZ) in area 17 displayed unique miRNA profiles. miRNAs present in primate, but absent in rodent, contributed disproportionately to the differential expression between GZ subregions. Prominent among the validated targets of these miRNAs were cell-cycle and neurogenesis regulators. Coevolution between the emergent miRNAs and their targets suggested that novel miRNAs became integrated into ancient gene circuitry to exert additional control over proliferation. We conclude that multiple cell-cycle regulatory events contribute to the emergence of primate-specific cortical features, including the OSVZ, generated enlarged supragranular layers, largely responsible for the increased primate cortex computational abilities.
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U2 - 10.1016/j.neuron.2014.01.017
DO - 10.1016/j.neuron.2014.01.017
M3 - Article
C2 - 24583023
AN - SCOPUS:84896314887
SN - 0896-6273
VL - 81
SP - 1255
EP - 1262
JO - Neuron
JF - Neuron
IS - 6
ER -