Novel Nanomonitor ultra-sensitive detection of troponin T

Thomas W. Barrett, Nandhinee Radha Shanmugam, Anjan Panneer Selvam, Steven (Steve) Kazmierczak, Shalini Prasad

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalClinica Chimica Acta
Volume442
DOIs
StatePublished - Mar 1 2015

Fingerprint

Troponin T
Biomarkers
Point-of-Care Systems
Troponin
Confidence Intervals
Equipment and Supplies
Acoustic impedance
Electric Impedance
Linear regression
Limit of Detection
Linear Models
Assays
Blood
Myocardial Infarction
Antibodies
Research

Keywords

  • Functional sensitivity
  • Nanotechnology
  • Point-of-care
  • Troponin T

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Novel Nanomonitor ultra-sensitive detection of troponin T. / Barrett, Thomas W.; Radha Shanmugam, Nandhinee; Selvam, Anjan Panneer; Kazmierczak, Steven (Steve); Prasad, Shalini.

In: Clinica Chimica Acta, Vol. 442, 01.03.2015, p. 96-101.

Research output: Contribution to journalArticle

Barrett, TW, Radha Shanmugam, N, Selvam, AP, Kazmierczak, SS & Prasad, S 2015, 'Novel Nanomonitor ultra-sensitive detection of troponin T', Clinica Chimica Acta, vol. 442, pp. 96-101. https://doi.org/10.1016/j.cca.2015.01.013
Barrett, Thomas W. ; Radha Shanmugam, Nandhinee ; Selvam, Anjan Panneer ; Kazmierczak, Steven (Steve) ; Prasad, Shalini. / Novel Nanomonitor ultra-sensitive detection of troponin T. In: Clinica Chimica Acta. 2015 ; Vol. 442. pp. 96-101.
@article{9187a0600da84b048159c7c828bd760d,
title = "Novel Nanomonitor ultra-sensitive detection of troponin T",
abstract = "Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38{\%} and 0.85{\%} at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10{\%} coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95{\%} confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95{\%} confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.",
keywords = "Functional sensitivity, Nanotechnology, Point-of-care, Troponin T",
author = "Barrett, {Thomas W.} and {Radha Shanmugam}, Nandhinee and Selvam, {Anjan Panneer} and Kazmierczak, {Steven (Steve)} and Shalini Prasad",
year = "2015",
month = "3",
day = "1",
doi = "10.1016/j.cca.2015.01.013",
language = "English (US)",
volume = "442",
pages = "96--101",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",

}

TY - JOUR

T1 - Novel Nanomonitor ultra-sensitive detection of troponin T

AU - Barrett, Thomas W.

AU - Radha Shanmugam, Nandhinee

AU - Selvam, Anjan Panneer

AU - Kazmierczak, Steven (Steve)

AU - Prasad, Shalini

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.

AB - Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.

KW - Functional sensitivity

KW - Nanotechnology

KW - Point-of-care

KW - Troponin T

UR - http://www.scopus.com/inward/record.url?scp=84922032860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922032860&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2015.01.013

DO - 10.1016/j.cca.2015.01.013

M3 - Article

C2 - 25619774

AN - SCOPUS:84922032860

VL - 442

SP - 96

EP - 101

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

ER -