Abstract
Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 96-101 |
| Number of pages | 6 |
| Journal | Clinica Chimica Acta |
| Volume | 442 |
| DOIs | |
| State | Published - Mar 1 2015 |
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Keywords
- Functional sensitivity
- Nanotechnology
- Point-of-care
- Troponin T
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Biochemistry, medical
Cite this
Novel Nanomonitor ultra-sensitive detection of troponin T. / Barrett, Thomas W.; Radha Shanmugam, Nandhinee; Selvam, Anjan Panneer; Kazmierczak, Steven (Steve); Prasad, Shalini.
In: Clinica Chimica Acta, Vol. 442, 01.03.2015, p. 96-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel Nanomonitor ultra-sensitive detection of troponin T
AU - Barrett, Thomas W.
AU - Radha Shanmugam, Nandhinee
AU - Selvam, Anjan Panneer
AU - Kazmierczak, Steven (Steve)
AU - Prasad, Shalini
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.
AB - Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.
KW - Functional sensitivity
KW - Nanotechnology
KW - Point-of-care
KW - Troponin T
UR - http://www.scopus.com/inward/record.url?scp=84922032860&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922032860&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2015.01.013
DO - 10.1016/j.cca.2015.01.013
M3 - Article
C2 - 25619774
AN - SCOPUS:84922032860
VL - 442
SP - 96
EP - 101
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -