Novel Nanomonitor ultra-sensitive detection of troponin T

Thomas W. Barrett, Nandhinee Radha Shanmugam, Anjan Panneer Selvam, Steven C. Kazmierczak, Shalini Prasad

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker. Methods: Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method. Results: The limit of detection of the Nanomonior for troponin T was 0.0088. ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73. ng/l and 1800. ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329. ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r = 1.00, and an intercept of 15.88. ng/l (95% confidence interval, - 68.39. ng/l, 100.15. ng/l). The mean difference between the assays was - 7.54. ng/l, determined by Bland-Altman analysis. Conclusion: The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15. min, and are portable. More research is needed.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalClinica Chimica Acta
Volume442
DOIs
StatePublished - Mar 1 2015

Keywords

  • Functional sensitivity
  • Nanotechnology
  • Point-of-care
  • Troponin T

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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