Novel Genetic Variants Associated With Increased Vertebral Volumetric BMD, Reduced Vertebral Fracture Risk, and Increased Expression of SLC1A3 and EPHB2

Carrie M. Nielson, Ching Ti Liu, Albert V. Smith, Cheryl L. Ackert-Bicknell, Sjur Reppe, Johanna Jakobsdottir, Christina Wassel, Thomas C. Register, Ling Oei, Nerea Alonso, Edwin H. Oei, Neeta Parimi, Elizabeth J. Samelson, Mike A. Nalls, Joseph Zmuda, Thomas Lang, Mary Bouxsein, Jeanne Latourelle, Melina Claussnitzer, Kristin SiggeirsdottirPriya Srikanth, Erik Lorentzen, Liesbeth Vandenput, Carl Langefeld, Laura Raffield, Greg Terry, Amanda J. Cox, Matthew A. Allison, Michael H. Criqui, Don Bowden, M. Arfan Ikram, Dan Mellström, Magnus K. Karlsson, John Carr, Matthew Budoff, Caroline Phillips, L. Adrienne Cupples, Wen Chi Chou, Richard H. Myers, Stuart H. Ralston, Kaare M. Gautvik, Peggy M. Cawthon, Steven Cummings, David Karasik, Fernando Rivadeneira, Vilmundur Gudnason, Eric S. Orwoll, Tamara B. Harris, Claes Ohlsson, Douglas P. Kiel, Yi Hsiang Hsu

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Genome-wide association studies (GWASs) have revealed numerous loci for areal bone mineral density (aBMD). We completed the first GWAS meta-analysis (n = 15,275) of lumbar spine volumetric BMD (vBMD) measured by quantitative computed tomography (QCT), allowing for examination of the trabecular bone compartment. SNPs that were significantly associated with vBMD were also examined in two GWAS meta-analyses to determine associations with morphometric vertebral fracture (n = 21,701) and clinical vertebral fracture (n = 5893). Expression quantitative trait locus (eQTL) analyses of iliac crest biopsies were performed in 84 postmenopausal women, and murine osteoblast expression of genes implicated by eQTL or by proximity to vBMD-associated SNPs was examined. We identified significant vBMD associations with five loci, including: 1p36.12, containing WNT4 and ZBTB40; 8q24, containing TNFRSF11B; and 13q14, containing AKAP11 and TNFSF11. Two loci (5p13 and 1p36.12) also contained associations with radiographic and clinical vertebral fracture, respectively. In 5p13, rs2468531 (minor allele frequency [MAF] = 3%) was associated with higher vBMD (β = 0.22, p = 1.9 × 10–8) and decreased risk of radiographic vertebral fracture (odds ratio [OR] = 0.75; false discovery rate [FDR] p = 0.01). In 1p36.12, rs12742784 (MAF = 21%) was associated with higher vBMD (β = 0.09, p = 1.2 × 10–10) and decreased risk of clinical vertebral fracture (OR = 0.82; FDR p = 7.4 × 10–4). Both SNPs are noncoding and were associated with increased mRNA expression levels in human bone biopsies: rs2468531 with SLC1A3 (β = 0.28, FDR p = 0.01, involved in glutamate signaling and osteogenic response to mechanical loading) and rs12742784 with EPHB2 (β = 0.12, FDR p = 1.7 × 10–3, functions in bone-related ephrin signaling). Both genes are expressed in murine osteoblasts. This is the first study to link SLC1A3 and EPHB2 to clinically relevant vertebral osteoporosis phenotypes. These results may help elucidate vertebral bone biology and novel approaches to reducing vertebral fracture incidence.

Original languageEnglish (US)
Pages (from-to)2085-2097
Number of pages13
JournalJournal of Bone and Mineral Research
Volume31
Issue number12
DOIs
StatePublished - Dec 1 2016

Keywords

  • ANALYSIS/QUANTITATION OF BONE
  • BONE QCT/μCT
  • DISEASES AND DISORDERS OF/RELATED TO BONE
  • EPIDEMIOLOGY, HUMAN ASSOCIATION STUDIES
  • FRACTURE RISK ASSESSMENT
  • GENERAL POPULATION STUDIES
  • GENETIC RESEARCH
  • OSTEOPOROSIS

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

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    Nielson, C. M., Liu, C. T., Smith, A. V., Ackert-Bicknell, C. L., Reppe, S., Jakobsdottir, J., Wassel, C., Register, T. C., Oei, L., Alonso, N., Oei, E. H., Parimi, N., Samelson, E. J., Nalls, M. A., Zmuda, J., Lang, T., Bouxsein, M., Latourelle, J., Claussnitzer, M., ... Hsu, Y. H. (2016). Novel Genetic Variants Associated With Increased Vertebral Volumetric BMD, Reduced Vertebral Fracture Risk, and Increased Expression of SLC1A3 and EPHB2. Journal of Bone and Mineral Research, 31(12), 2085-2097. https://doi.org/10.1002/jbmr.2913