Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication

Won Chung; Wonhee Lee; John T. Loffredo; Benjamin Burwitz; Thomas C. Friedrich; Juan Pablo Giraldo Vela; Gnankang Napoe; Eva G. Rakasz; Nancy A. Wilson; David B. Allison; David I. Watkins

doi:10.1128/JVI.01780-06

Not all cytokine-producing CD8⁺ T cells suppress simian immunodeficiency virus replication

Won Chung, Wonhee Lee, John T. Loffredo, Benjamin Burwitz, Thomas C. Friedrich, Juan Pablo Giraldo Vela, Gnankang Napoe, Eva G. Rakasz, Nancy A. Wilson, David B. Allison, David I. Watkins

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Current assays of CD8⁺ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8⁺ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef _165-173IW9- and Vif^66-73HW8-speciflc clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.

Original language	English (US)
Pages (from-to)	1517-1523
Number of pages	7
Journal	Journal of virology
Volume	81
Issue number	3
DOIs	https://doi.org/10.1128/JVI.01780-06
State	Published - Feb 2007
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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title = "Not all cytokine-producing CD8+ T cells suppress simian immunodeficiency virus replication",

abstract = "Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef 165-173IW9- and Vif66-73HW8-speciflc clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.",

author = "Won Chung and Wonhee Lee and Loffredo, {John T.} and Benjamin Burwitz and Friedrich, {Thomas C.} and Vela, {Juan Pablo Giraldo} and Gnankang Napoe and Rakasz, {Eva G.} and Wilson, {Nancy A.} and Allison, {David B.} and Watkins, {David I.}",

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AU - Chung, Won

AU - Lee, Wonhee

AU - Loffredo, John T.

AU - Burwitz, Benjamin

AU - Friedrich, Thomas C.

AU - Vela, Juan Pablo Giraldo

AU - Napoe, Gnankang

AU - Rakasz, Eva G.

AU - Wilson, Nancy A.

AU - Allison, David B.

AU - Watkins, David I.

PY - 2007/2

Y1 - 2007/2

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AB - Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef 165-173IW9- and Vif66-73HW8-speciflc clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.

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Not all cytokine-producing CD8⁺ T cells suppress simian immunodeficiency virus replication

Abstract

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