Nosocomial outbreak of extensively drug-resistant acinetobacter baumannii isolates containing blaOXA-237 carried on a plasmid

Andrea M. Hujer, Paul G. Higgins, Susan D. Rudin, Genevieve L. Buser, Steven H. Marshall, Kyriaki Xanthopoulou, Harald Seifert, Laura J. Rojas, T. Nicholas Domitrovic, P. Maureen Cassidy, Margaret C. Cunningham, Robert Vega, Jon P. Furuno, Christopher Pfeiffer, Zintars G. Beldavs, Meredith S. Wright, Michael R. Jacobs, Mark D. Adams, Robert A. Bonomo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Carbapenem antibiotics are among the mainstays for treating infections caused by Acinetobacter baumannii, especially in the Northwest United States, where carbapenem-resistant A. baumannii remains relatively rare. However, between June 2012 and October 2014, an outbreak of carbapenem-resistant A. baumannii occurred in 16 patients from five health care facilities in the state of Oregon. All isolates were defined as extensively drug resistant. Multilocus sequence typing revealed that the isolates belonged to sequence type 2 (international clone 2 [IC2]) and were 95% similar as determined by repetitive-sequence-based PCR analysis. Multiplex PCR revealed the presence of a blaOXA carbapenemase gene, later identified as blaOXA-237. Whole-genome sequencing of all isolates revealed a well-supported separate branch within a global A. baumannii phylogeny. Pacific Biosciences (PacBio) SMRT sequencing was also performed on one isolate to gain insight into the genetic location of the carbapenem resistance gene. We discovered that blaOXA-237, flanked on either side by ISAba1 elements in opposite orientations, was carried on a 15,198-bp plasmid designated pORAB01-3 and was present in all 16 isolates. The plasmid also contained genes encoding a TonB-dependent receptor, septicolysin, a type IV secretory pathway (VirD4 component, TraG/TraD family) ATPase, an integrase, a RepB family plasmid DNA replication initiator protein, an alpha/beta hydrolase, and a BrnT/BrnA type II toxin-antitoxin system. This is the first reported outbreak in the northwestern United States associated with this carbapenemase. Particularly worrisome is that blaOXA-237 was carried on a plasmid and found in the most prominent worldwide clonal group IC2, potentially giving pORAB01-3 great capacity for future widespread dissemination.

Original languageEnglish (US)
Article numbere00797-17
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Acinetobacter baumannii
Carbapenems
Disease Outbreaks
Plasmids
Pharmaceutical Preparations
Clone Cells
Northwestern United States
Genes
Antitoxins
Multilocus Sequence Typing
Integrases
Nucleic Acid Repetitive Sequences
Secretory Pathway
Multiplex Polymerase Chain Reaction
Health Facilities
Hydrolases
Phylogeny
DNA Replication
Adenosine Triphosphatases
Genome

Keywords

  • Acinetobacter
  • Acinetobacter baumannii
  • Carbapenemase
  • OXA-237
  • Plasmid

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Hujer, A. M., Higgins, P. G., Rudin, S. D., Buser, G. L., Marshall, S. H., Xanthopoulou, K., ... Bonomo, R. A. (2017). Nosocomial outbreak of extensively drug-resistant acinetobacter baumannii isolates containing blaOXA-237 carried on a plasmid. Antimicrobial Agents and Chemotherapy, 61(11), [e00797-17]. https://doi.org/10.1128/AAC.00797-17

Nosocomial outbreak of extensively drug-resistant acinetobacter baumannii isolates containing blaOXA-237 carried on a plasmid. / Hujer, Andrea M.; Higgins, Paul G.; Rudin, Susan D.; Buser, Genevieve L.; Marshall, Steven H.; Xanthopoulou, Kyriaki; Seifert, Harald; Rojas, Laura J.; Domitrovic, T. Nicholas; Cassidy, P. Maureen; Cunningham, Margaret C.; Vega, Robert; Furuno, Jon P.; Pfeiffer, Christopher; Beldavs, Zintars G.; Wright, Meredith S.; Jacobs, Michael R.; Adams, Mark D.; Bonomo, Robert A.

In: Antimicrobial Agents and Chemotherapy, Vol. 61, No. 11, e00797-17, 01.11.2017.

Research output: Contribution to journalArticle

Hujer, AM, Higgins, PG, Rudin, SD, Buser, GL, Marshall, SH, Xanthopoulou, K, Seifert, H, Rojas, LJ, Domitrovic, TN, Cassidy, PM, Cunningham, MC, Vega, R, Furuno, JP, Pfeiffer, C, Beldavs, ZG, Wright, MS, Jacobs, MR, Adams, MD & Bonomo, RA 2017, 'Nosocomial outbreak of extensively drug-resistant acinetobacter baumannii isolates containing blaOXA-237 carried on a plasmid', Antimicrobial Agents and Chemotherapy, vol. 61, no. 11, e00797-17. https://doi.org/10.1128/AAC.00797-17
Hujer, Andrea M. ; Higgins, Paul G. ; Rudin, Susan D. ; Buser, Genevieve L. ; Marshall, Steven H. ; Xanthopoulou, Kyriaki ; Seifert, Harald ; Rojas, Laura J. ; Domitrovic, T. Nicholas ; Cassidy, P. Maureen ; Cunningham, Margaret C. ; Vega, Robert ; Furuno, Jon P. ; Pfeiffer, Christopher ; Beldavs, Zintars G. ; Wright, Meredith S. ; Jacobs, Michael R. ; Adams, Mark D. ; Bonomo, Robert A. / Nosocomial outbreak of extensively drug-resistant acinetobacter baumannii isolates containing blaOXA-237 carried on a plasmid. In: Antimicrobial Agents and Chemotherapy. 2017 ; Vol. 61, No. 11.
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