Normothermic cardiac arrest and cardiopulmonary resuscitation

a mouse model of ischemia-reperfusion injury.

Michael Hutchens, Richard J. Traystman, Tetsuhiro Fujiyoshi, Shin Nakayama, Paco S. Herson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number54
StatePublished - 2011

Fingerprint

Resuscitation
Cardiopulmonary Resuscitation
Heart Arrest
Reperfusion Injury
Acute Kidney Injury
Anatomic Models
Genetic Techniques
Critical Illness
Reproduction
Rodentia
Animals
Ischemia
Animal Models
Costs and Cost Analysis
Mortality

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Normothermic cardiac arrest and cardiopulmonary resuscitation : a mouse model of ischemia-reperfusion injury. / Hutchens, Michael; Traystman, Richard J.; Fujiyoshi, Tetsuhiro; Nakayama, Shin; Herson, Paco S.

In: Journal of visualized experiments : JoVE, No. 54, 2011.

Research output: Contribution to journalArticle

@article{01d2889babf4426cbd644405ffcdb0a8,
title = "Normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury.",
abstract = "Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.",
author = "Michael Hutchens and Traystman, {Richard J.} and Tetsuhiro Fujiyoshi and Shin Nakayama and Herson, {Paco S.}",
year = "2011",
language = "English (US)",
journal = "Journal of visualized experiments : JoVE",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "54",

}

TY - JOUR

T1 - Normothermic cardiac arrest and cardiopulmonary resuscitation

T2 - a mouse model of ischemia-reperfusion injury.

AU - Hutchens, Michael

AU - Traystman, Richard J.

AU - Fujiyoshi, Tetsuhiro

AU - Nakayama, Shin

AU - Herson, Paco S.

PY - 2011

Y1 - 2011

N2 - Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

AB - Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality and which is most frequently caused by whole-body hypoperfusion. Successful reproduction of whole-body hypoperfusion in rodent models has been fraught with difficulty. Models which employ focal ischemia have repeatedly demonstrated results which do not translate to the clinical setting, and larger animal models which allow for whole body hypoperfusion lack access to the full toolset of genetic manipulation possible in the mouse. However, in recent years a mouse model of cardiac arrest and cardiopulmonary resuscitation has emerged which can be adapted to model AKI. This model reliably reproduces physiologic, functional, anatomic, and histologic outcomes seen in clinical AKI, is rapidly repeatable, and offers all of the significant advantages of a murine surgical model, including access to genetic manipulative techniques, low cost relative to large animals, and ease of use. Our group has developed extensive experience with use of this model to assess a number of organ-specific outcomes in AKI.

UR - http://www.scopus.com/inward/record.url?scp=84859926211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859926211&partnerID=8YFLogxK

M3 - Article

JO - Journal of visualized experiments : JoVE

JF - Journal of visualized experiments : JoVE

SN - 1940-087X

IS - 54

ER -