Nonspecific cleavage of φX174 RFI deoxyribonucleic acid by bleomycin

Jack D. Love, Charles D. Liarakos, Robb Moses

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The covalently closed circular duplex deoxyribonucleic acid (DNA) of φX174 underwent progressive conversion to nicked and linear DNA with increasing bleomycin/φX174 RFI DNA molecule ratios. The formation of linear DNA (a double-strand break) occurred under limited reaction conditions as low as an average of 0.2 single-strand break/φX174 RFI DNA molecule. As bleomycin-produced linear DNA was further fragmented by bleomycin, a broad distribution of DNA fragments without notable concentrations of unique size was formed. Restriction enzymes PstI and SstII did not generate discrete fragments from bleomycin-produced full-length linear φX174 DNA, nor did bleomycin cleavage generate discrete fragments from HpaII or PstI digests of φX174 RFI. These findings suggest that bleomycin does not act at a few specific sites on φX174 RFI DNA. The single-strand nick appeared to be the preferred site for bleomycin action for a second cleavage in a φX174 molecule.

Original languageEnglish (US)
Pages (from-to)5331-5336
Number of pages6
JournalBiochemistry
Volume20
Issue number18
StatePublished - 1981
Externally publishedYes

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Bleomycin
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  • Biochemistry

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Nonspecific cleavage of φX174 RFI deoxyribonucleic acid by bleomycin. / Love, Jack D.; Liarakos, Charles D.; Moses, Robb.

In: Biochemistry, Vol. 20, No. 18, 1981, p. 5331-5336.

Research output: Contribution to journalArticle

Love, JD, Liarakos, CD & Moses, R 1981, 'Nonspecific cleavage of φX174 RFI deoxyribonucleic acid by bleomycin', Biochemistry, vol. 20, no. 18, pp. 5331-5336.
Love, Jack D. ; Liarakos, Charles D. ; Moses, Robb. / Nonspecific cleavage of φX174 RFI deoxyribonucleic acid by bleomycin. In: Biochemistry. 1981 ; Vol. 20, No. 18. pp. 5331-5336.
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