Noninvasive ultrasound imaging of inflammation using microbubbles targeted to activated leukocytes

Jonathan Lindner, J. Song, F. Xu, A. L. Klibanov, K. Singbartl, K. Ley, Sanjiv Kaul

Research output: Contribution to journalArticle

247 Scopus citations

Abstract

Background - Lipid microbubbles used for perfusion imaging with ultrasound are retained within inflamed tissue because of complement-mediated attachment to leukocytes within venules. We hypothesized that incorporation of phosphatidylserine (PS) into the microbubble shell may enhance these interactions by amplifying complement activation and thereby allow ultrasound imaging of inflammation. Methods and Results - In 6 mice, intravital microscopy of tissue necrosis factor-α-treated cremaster muscle was performed to assess the microvascular behavior of fluorescein-labeled lipid microbubbles with and without PS in the shell. Ten minutes after intravenous injection, microbubble attachment to leukocytes within inflamed venules was greater for PS-containing than for standard lipid microbubbles (20±4 versus 10±3 per 20 optical fields, P

Original languageEnglish (US)
Pages (from-to)2745-2750
Number of pages6
JournalCirculation
Volume102
Issue number22
Publication statusPublished - Nov 28 2000
Externally publishedYes

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Keywords

  • Echocardiography
  • Inflammation
  • Leukocytes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Lindner, J., Song, J., Xu, F., Klibanov, A. L., Singbartl, K., Ley, K., & Kaul, S. (2000). Noninvasive ultrasound imaging of inflammation using microbubbles targeted to activated leukocytes. Circulation, 102(22), 2745-2750.