Noninvasive Topical In Vivo Imaging of Skin: Confocal Reflectance Microscopy and Polarized Light Imaging

Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Topical, noninvasive, label-free imaging of skin can be accomplished using a variety of techniques. This chapter discusses two methods that are sensitive to the structure of tissue on the 10-10-μm scale because they are based on light scattering, as opposed to methods, such as fluorescence and Raman spectroscopy that inform about the chemical composition of a tissue. The two methods are: (1) confocal reflectance imaging that includes both confocal scanning laser microscopy (CSLM) and optical coherence tomography (OCT), and (2) polarized light imaging, which is sensitive to both light-scattering structures and birefringent fibers like collagen and actin-myosin.

Original languageEnglish (US)
Title of host publicationImaging in Dermatology
PublisherElsevier Inc.
Number of pages10
ISBN (Print)9780128028384
Publication statusPublished - Aug 19 2016



  • Confocal reflectance (CR)
  • Confocal scanning laser microscopy (CSLM)
  • Optical coherence tomography (OCT)
  • Optical scattering

ASJC Scopus subject areas

  • Medicine(all)

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