Noninvasive murine glioma detection improved following photobleaching of skin PpIX fluorescence

Summer L. Gibbs-Strauss, Scott C. Davis, Julia A. O'Hara, P. Jack Hoopes, Tayyaba Hasan, Brian W. Pogue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Aminolevulinic Acid (ALA) is a prodrug which can be administered to cells, animals or patients after which it is transformed via the Heme synthesis pathway into the fluorescent molecule Protoporphyrin IX (PpIX). PpIX has been shown to be useful as both a photosensitizer for photodynamic therapy (PDT) and as a fluorescence imaging contrast agent. The ALA-PpIX system not only provides contrast for fluorescence imaging but also gives information about the metabolic activity of the imaged tissue and thus could be useful for monitoring cancer therapy. In the current study skin photobleaching was examined to determine if PpIX fluorescence contrast in malignant brain tumors could be better visualized noninvasively. Red light photobleaching decreased skin PpIX fluorescence and increased the ability to noninvasively quantify PpIX fluorescence in murine gliomas, as in vivo measurements of mean PpIX fluorescence more closely matched ex vivo quantification following skin photobleaching. Three doses of blue light photobleaching (4 J/cm2, 8 J/cm2 and 12 J/cm2) were tested and determined to give similar levels of skin photobleaching as well as a similar window of decreased skin PpIX fluorescence for noninvasive fluorescence imaging following the photobleaching dose administration.

Original languageEnglish (US)
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XVII
DOIs
StatePublished - Apr 21 2008
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII - San Jose, CA, United States
Duration: Jan 19 2008Jan 20 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6845
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII
CountryUnited States
CitySan Jose, CA
Period1/19/081/20/08

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Gibbs-Strauss, S. L., Davis, S. C., O'Hara, J. A., Hoopes, P. J., Hasan, T., & Pogue, B. W. (2008). Noninvasive murine glioma detection improved following photobleaching of skin PpIX fluorescence. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII [68450D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6845). https://doi.org/10.1117/12.762997