Noninvasive measurement of aminolevulinic acid-induced protoporphyrin IX fluorescence allowing detection of murine glioma in vivo

Summer Gibbs, Julia A. O'Hara, P. Jack Hoopes, Tayyaba Hasan, Brian W. Pogue

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence is studied as a contrast agent for noninvasive detection of murine glioma, using the fluorescence-to-transmission ratio measured through the cranium. Signals measured prior to administration of ALA are very similar between control animals, 9L-GFP, and U251 tumor-bearing animals. However, 2h after ALA administration, the PpIX signal from both tumor-bearing groups is significantly higher than the control group (9L-GFP group p-value=0.016, and U251 group p-value=0.004, relative to the control group). The variance in signal from the 9L-GFP group is much larger than either the control group or the U251 group, which is consistent with higher intrinsic PpIX fluorescence heterogeneity as seen in situ at ex vivo analysis. Decreasing the skin PpIX fluorescence via intentional photobleaching using red light (635nm) is examined as a tool for increasing PpIX contrast between the tumor-bearing and control groups. The red light bleaching is found to increase the ability to accurately quantify PpIX fluorescence in vivo, but decreases the specificity of detection between tumor-bearing and nontumor-bearing groups.

Original languageEnglish (US)
Article number014007
JournalJournal of Biomedical Optics
Volume14
Issue number1
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Bearings (structural)
Aminolevulinic Acid
Fluorescence
Tumors
fluorescence
acids
Acids
Animals
tumors
Photobleaching
Bleaching
animals
Skin
cranium
Contrast Media
protoporphyrin IX
bleaching

Keywords

  • aminolevulinic acid
  • fluorescenace
  • glioma
  • protoporphyrin IX
  • spectroscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Noninvasive measurement of aminolevulinic acid-induced protoporphyrin IX fluorescence allowing detection of murine glioma in vivo. / Gibbs, Summer; O'Hara, Julia A.; Hoopes, P. Jack; Hasan, Tayyaba; Pogue, Brian W.

In: Journal of Biomedical Optics, Vol. 14, No. 1, 014007, 2009.

Research output: Contribution to journalArticle

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