TY - JOUR
T1 - Noninvasive imaging of melanoma with reflectance mode confocal scanning laser microscopy in a murine model
AU - Gareau, Daniel S.
AU - Merlino, Glenn
AU - Corless, Christopher
AU - Kulesz-Martin, Molly
AU - Jacques, Steven L.
N1 - Funding Information:
This work was supported by the National Institutes of Health, EB000224 (SLJ), CA98893 (MKM), and CA69533 (OHSU Cancer Institute). We thank Drs. Alon Scope, Frances Noonan, Ed De Fabo, and Miriam Anver for critical review of this paper. We also thank Dr. Miriam Anver and Carolyn Gendron for histopathology.
PY - 2007/9
Y1 - 2007/9
N2 - A reflectance-mode confocal scanning laser microscope (rCSLM) was developed for imaging early-stage melanoma in a living mouse model without the addition of exogenous contrast agents. Lesions were first located by surveying the dorsum with a polarized light camera, then imaged with the rCSLM. The images demonstrated two characteristics of melanoma in this animal model: (1) melanocytes and apparent tumor nests in the epidermis at the stratum spinosum in a state of pagetoid spread and (2) architectural disruption of the dermal-epidermal junction. The epidermal melanocytes and apparent tumor nests had a high melanin content, which caused their reflectance to be fivefold greater than the surrounding epidermis. The rCSLM images illustrate the difference between normal skin and sites with apparent melanoma. This imaging modality shows promise to track the progression of melanoma lesions in animal models.
AB - A reflectance-mode confocal scanning laser microscope (rCSLM) was developed for imaging early-stage melanoma in a living mouse model without the addition of exogenous contrast agents. Lesions were first located by surveying the dorsum with a polarized light camera, then imaged with the rCSLM. The images demonstrated two characteristics of melanoma in this animal model: (1) melanocytes and apparent tumor nests in the epidermis at the stratum spinosum in a state of pagetoid spread and (2) architectural disruption of the dermal-epidermal junction. The epidermal melanocytes and apparent tumor nests had a high melanin content, which caused their reflectance to be fivefold greater than the surrounding epidermis. The rCSLM images illustrate the difference between normal skin and sites with apparent melanoma. This imaging modality shows promise to track the progression of melanoma lesions in animal models.
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U2 - 10.1038/sj.jid.5700829
DO - 10.1038/sj.jid.5700829
M3 - Article
C2 - 17460734
AN - SCOPUS:34547854315
SN - 0022-202X
VL - 127
SP - 2184
EP - 2190
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 9
ER -