Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles

Jonathan Lindner, Paul A. Dayton, Matthew P. Coggins, Klaus Ley, Ji Song, Katherine Ferrara, Sanjiv Kaul

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Background - We have previously shown that microbubbles adhere to leukocytes in regions of inflammation. We hypothesized that these microbubbles are phagocytosed by neutrophils and monocytes and remain acoustically active, permitting their detection in inflamed tissue. Methods and Results - In vitro studies were performed in which activated leukocytes were incubated with albumin or lipid microbubbles and observed under microscopy. Microbubbles attached to the surface of activated neutrophils and monocytes, were phagocytosed, and remained intact for up to 30 minutes. The rate of destruction of the phagocytosed microbubbles on exposure to ultrasound was less (P ≤ 0.05) than that of free microbubbles at all acoustic pressures applied. Intravital microscopy and simultaneous ultrasound imaging of the cremaster muscle was performed in 6 mice to determine whether phagocytosed microbubbles could be detected in vivo. Fifteen minutes after intravenous injection of fluorescein-labeled microbubbles, when the blood-pool concentration was negligible, the number of phagocytosed/attached microbubbles within venules was 7-fold greater in tumor necrosis factor-α (TNF-α)-treated animals than in control animals (P <0.01). This increase in retained microbubbles resulted in a 5- to 6-fold-greater (P <0.01) degree of ultrasound contrast enhancement than in controls. Conclusions - After attaching to activated neutrophils and monocytes, microbubbles are phagocytosed intact. Despite viscoelastic damping, phagocytosed microbubbles remain responsive to ultrasound and can be detected by ultrasound in vivo after clearance of freely circulating microbubbles from the blood pool. Thus, contrast ultrasound has potential for imaging sites of inflammation.

Original languageEnglish (US)
Pages (from-to)531-538
Number of pages8
JournalCirculation
Volume102
Issue number5
StatePublished - Aug 1 2000
Externally publishedYes

Fingerprint

Microbubbles
Phagocytosis
Ultrasonography
Inflammation
Monocytes
Neutrophils
Leukocytes
Abdominal Muscles
Venules
Fluorescein
Acoustics
Intravenous Injections
Microscopy
Albumins

Keywords

  • Imaging
  • Inflammation
  • Leukocytes
  • Ultrasonics

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Lindner, J., Dayton, P. A., Coggins, M. P., Ley, K., Song, J., Ferrara, K., & Kaul, S. (2000). Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles. Circulation, 102(5), 531-538.

Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles. / Lindner, Jonathan; Dayton, Paul A.; Coggins, Matthew P.; Ley, Klaus; Song, Ji; Ferrara, Katherine; Kaul, Sanjiv.

In: Circulation, Vol. 102, No. 5, 01.08.2000, p. 531-538.

Research output: Contribution to journalArticle

Lindner, J, Dayton, PA, Coggins, MP, Ley, K, Song, J, Ferrara, K & Kaul, S 2000, 'Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles', Circulation, vol. 102, no. 5, pp. 531-538.
Lindner J, Dayton PA, Coggins MP, Ley K, Song J, Ferrara K et al. Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles. Circulation. 2000 Aug 1;102(5):531-538.
Lindner, Jonathan ; Dayton, Paul A. ; Coggins, Matthew P. ; Ley, Klaus ; Song, Ji ; Ferrara, Katherine ; Kaul, Sanjiv. / Noninvasive imaging of inflammation by ultrasound detection of Phagocytosed microbubbles. In: Circulation. 2000 ; Vol. 102, No. 5. pp. 531-538.
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AB - Background - We have previously shown that microbubbles adhere to leukocytes in regions of inflammation. We hypothesized that these microbubbles are phagocytosed by neutrophils and monocytes and remain acoustically active, permitting their detection in inflamed tissue. Methods and Results - In vitro studies were performed in which activated leukocytes were incubated with albumin or lipid microbubbles and observed under microscopy. Microbubbles attached to the surface of activated neutrophils and monocytes, were phagocytosed, and remained intact for up to 30 minutes. The rate of destruction of the phagocytosed microbubbles on exposure to ultrasound was less (P ≤ 0.05) than that of free microbubbles at all acoustic pressures applied. Intravital microscopy and simultaneous ultrasound imaging of the cremaster muscle was performed in 6 mice to determine whether phagocytosed microbubbles could be detected in vivo. Fifteen minutes after intravenous injection of fluorescein-labeled microbubbles, when the blood-pool concentration was negligible, the number of phagocytosed/attached microbubbles within venules was 7-fold greater in tumor necrosis factor-α (TNF-α)-treated animals than in control animals (P <0.01). This increase in retained microbubbles resulted in a 5- to 6-fold-greater (P <0.01) degree of ultrasound contrast enhancement than in controls. Conclusions - After attaching to activated neutrophils and monocytes, microbubbles are phagocytosed intact. Despite viscoelastic damping, phagocytosed microbubbles remain responsive to ultrasound and can be detected by ultrasound in vivo after clearance of freely circulating microbubbles from the blood pool. Thus, contrast ultrasound has potential for imaging sites of inflammation.

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