Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid

Jane Hitti, Jodi Lapidus, Xinfang Lu, Ashok P. Reddy, Thomas Jacob, Surendra Dasari, David A. Eschenbach, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor. Study Design: Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression. Results: Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection. Conclusion: Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number1
DOIs
StatePublished - Jul 2010

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Proteomics
Biomarkers
Proteome
Infection
Premature Obstetric Labor
Amniotic Fluid
Immunoenzyme Techniques
Proteins
Acute-Phase Proteins
Extracellular Matrix Proteins
Tandem Mass Spectrometry
Liquid Chromatography
Interleukin-6
Logistic Models

Keywords

  • intraamniotic infection
  • preterm labor
  • proteomics
  • vaginal fluid

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Noninvasive diagnosis of intraamniotic infection : proteomic biomarkers in vaginal fluid. / Hitti, Jane; Lapidus, Jodi; Lu, Xinfang; Reddy, Ashok P.; Jacob, Thomas; Dasari, Surendra; Eschenbach, David A.; Gravett, Michael G.; Nagalla, Srinivasa R.

In: American Journal of Obstetrics and Gynecology, Vol. 203, No. 1, 07.2010.

Research output: Contribution to journalArticle

Hitti, Jane ; Lapidus, Jodi ; Lu, Xinfang ; Reddy, Ashok P. ; Jacob, Thomas ; Dasari, Surendra ; Eschenbach, David A. ; Gravett, Michael G. ; Nagalla, Srinivasa R. / Noninvasive diagnosis of intraamniotic infection : proteomic biomarkers in vaginal fluid. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 203, No. 1.
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