Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid

Jane Hitti, Jodi A. Lapidus, Xinfang Lu, Ashok P. Reddy, Thomas Jacob, Surendra Dasari, David A. Eschenbach, Michael G. Gravett, Srinivasa R. Nagalla

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Objective: We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor. Study Design: Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression. Results: Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection. Conclusion: Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.

Original languageEnglish (US)
Pages (from-to)32.e1-32.e8
JournalAmerican journal of obstetrics and gynecology
Volume203
Issue number1
DOIs
StatePublished - Jul 2010

Keywords

  • intraamniotic infection
  • preterm labor
  • proteomics
  • vaginal fluid

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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    Hitti, J., Lapidus, J. A., Lu, X., Reddy, A. P., Jacob, T., Dasari, S., Eschenbach, D. A., Gravett, M. G., & Nagalla, S. R. (2010). Noninvasive diagnosis of intraamniotic infection: proteomic biomarkers in vaginal fluid. American journal of obstetrics and gynecology, 203(1), 32.e1-32.e8. https://doi.org/10.1016/j.ajog.2010.03.037