Nongenomic mechanisms of glucocorticoid inhibition of nicotine-induced calcium influx in PC12 cells: Involvement of protein kinase C

Jian Qiu, Li Guang Lou, Xiu Ying Huang, Shu Jie Lou, Gang Pei, Yi Zhang Chen

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Nongenomic mechanisms of corticosterone (B) inhibition of nicotine (Nic)-induced calcium influx were investigated in PC12 cells. Corticosterone could rapidly inhibit the Ca2+ influx induced by Nic, and BSA-conjugated B had a similar inhibitory effect. The inhibition of Nic-induced Ca2+ influx by B could be mimicked by protein kinase C (PKC) activator (phorbol 12- myristate 13-acetate) and reversed by PKC inhibitors, chelerythrine chloride and Go6976. When PC 12 cells were pretreated with pertussis toxin, the inhibitory effect of B on Nic-induced Ca2+ influx was blocked. Both B and BSA-conjugated B could activate PKC activity, with the maximal responses at 10-9 and 10-7 M at 37 C, respectively. The dose-response curve was bell shaped. At 25 C, however, the dose-response curve considerably shifted to the right, and B was most potent at 10-5 M. The time course showed that PKC activity was highest at 5 min of B's action. The results suggest that B might act via putative membrane receptors and inhibit the Ca2+ influx induced by Nic through the pertussis toxin-sensitive G protein-PKC pathway and that PKC plays an important role in the mechanisms of glucocorticoid nongenomic action.

Original languageEnglish (US)
Pages (from-to)5103-5108
Number of pages6
JournalEndocrinology
Volume139
Issue number12
StatePublished - 1998
Externally publishedYes

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PC12 Cells
Nicotine
Protein Kinase C
Glucocorticoids
Calcium
Pertussis Toxin
Corticosterone
Protein C Inhibitor
Protein Kinase Inhibitors
GTP-Binding Proteins
Acetates
Membranes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Nongenomic mechanisms of glucocorticoid inhibition of nicotine-induced calcium influx in PC12 cells : Involvement of protein kinase C. / Qiu, Jian; Lou, Li Guang; Huang, Xiu Ying; Lou, Shu Jie; Pei, Gang; Chen, Yi Zhang.

In: Endocrinology, Vol. 139, No. 12, 1998, p. 5103-5108.

Research output: Contribution to journalArticle

Qiu, Jian ; Lou, Li Guang ; Huang, Xiu Ying ; Lou, Shu Jie ; Pei, Gang ; Chen, Yi Zhang. / Nongenomic mechanisms of glucocorticoid inhibition of nicotine-induced calcium influx in PC12 cells : Involvement of protein kinase C. In: Endocrinology. 1998 ; Vol. 139, No. 12. pp. 5103-5108.
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