Nonequivalent requirements for PS1 and PS2 integrin at cell attachments in Drosophila: Genetic analysis of the α(PS1) integrin subunit

D. L. Brower, T. A. Bunch, L. Mukai, T. E. Adamson, M. Wehrli, S. Lam, E. Friedlander, C. E. Roote, S. Zusman

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

We report on the generation and phenotype of mutant alleles of multiple edematous wings (mew), the gene encoding the α(PS1) subunit of the PS1 integrin of Drosophila. None of the six alleles examined makes detectable protein, and one allele results from a chromosome break near the middle of the translated sequence, so we are confident that we have described the null phenotype. In contrast to if (α(PS2)) and mys (β(PS)) mutants, most mutant mew embryos hatch, to die as larvae. Mutant mew embryos display abnormal gut morphogenesis but, unlike mys or if embryos, there is no evidence of defects in the somatic muscles. Thus, the complementary distributions of PS1 (α(PS1)β(PS)) and PS2 (α(PS2)β(PS)) integrin on tendon cells and muscle, respectively, do not reflect equivalent requirements at the myotendinous junction. Dorsal herniation, characteristic of the mys lethal phenotype, is not observed in mew or in mew if embryos. Clonal analysis experiments indicate that eye morphogenesis is disrupted in mew clones, but if clones in the eye are relatively normal in morphology. Adult wings display blisters around large dorsal but not ventral mew clones. In contrast to dorsal mys clones, small mew patches do not necessarily display morphogenetic abnormalities. Thus, another integrin in addition to PS1 appears to function on the dorsal wing surface.

Original languageEnglish (US)
Pages (from-to)1311-1320
Number of pages10
JournalDevelopment
Volume121
Issue number5
StatePublished - Jan 1 1995

Keywords

  • Eye morphogenesis
  • Mew
  • Multiple edematous wings
  • Muscle attachment
  • PS integrin
  • Wing morphogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Brower, D. L., Bunch, T. A., Mukai, L., Adamson, T. E., Wehrli, M., Lam, S., Friedlander, E., Roote, C. E., & Zusman, S. (1995). Nonequivalent requirements for PS1 and PS2 integrin at cell attachments in Drosophila: Genetic analysis of the α(PS1) integrin subunit. Development, 121(5), 1311-1320.