Non-human primate models

Artificial menstrual cycles, endometrial matrix metalloproteinases and s.c. endometrial grafts

R. M. Brenner, L. Rudolph, L. Matrisian, Ov Slayden

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    Rhesus monkeys are useful models in which to examine the hormonal regulation of endometrial matrix metalloproteinases (MMP) and to evaluate the role of MMP in uterine bleeding. Artificial 28 day menstrual cycles can be induced in ovariectomized monkeys by inserting an oestradiol implant for 2 weeks, then inserting a progesterone implant for 2 weeks, and then, with the oestradiol implant remaining in place, removing and reinserting the progesterone implant at 2 week intervals. To examine MMP during menses, we established such cycles and removed uteri by hysterectomy at closely spaced intervals before, during and after menses, as well as at later time points. Some samples were also obtained during menses induced by the withdrawal of both progesterone and oestradiol. We examined mRNA of the following MMP by Northern blotting: matrilysin, stromelysin-1, stromelysin-2, stromelysin-3 and the tissue inhibitor of MMP TIMP-1. The expression of these MMP mRNA increased substantially by 2-3 days after progesterone withdrawal, whether or not oestradiol was maintained. The expression of some of the MMP (stromelysins-1 and -2) returned very rapidly to baseline levels by 5 days after progesterone withdrawal, while the expression of others (matrilysin, stromelysin-3 and TIMP-1) declined more slowly, reaching a baseline level by 10 days after progesterone withdrawal, with little or no further decline after progesterone concentrations rose during the induced luteal phase. Immunocytochemical studies showed that matrilysin was expressed primarily in the glands of the upper functionalis. In other work with the rhesus monkey model, we used a s.c. endometrial autograft technique in which pieces of endometrium were autotransplanted to the abdominal skin. During menses in the grafts, matrilysin was expressed in the glands of the grafts similar to the glands in the eutopic endometrium. Endometrial autografts can serve as a useful model for the study of MMP in uterine bleeding.

    Original languageEnglish (US)
    Pages (from-to)150-164
    Number of pages15
    JournalHuman Reproduction
    Volume11
    Issue numberSUPPL. 2
    StatePublished - 1996

    Fingerprint

    Menstrual Cycle
    Matrix Metalloproteinases
    Primates
    Progesterone
    Matrix Metalloproteinase 7
    Menstruation
    Transplants
    Estradiol
    Matrix Metalloproteinase 10
    Matrix Metalloproteinase 11
    Matrix Metalloproteinase 3
    Matrix Metalloproteinase 1
    Tissue Inhibitor of Metalloproteinase-1
    Uterine Hemorrhage
    Autografts
    Endometrium
    Macaca mulatta
    Tissue Inhibitor of Metalloproteinases
    Messenger RNA
    Luteal Phase

    Keywords

    • Autograft
    • Endometrium
    • Matrix metalloproteinases
    • Menstruation
    • Non-human primates

    ASJC Scopus subject areas

    • Physiology
    • Developmental Biology
    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    Non-human primate models : Artificial menstrual cycles, endometrial matrix metalloproteinases and s.c. endometrial grafts. / Brenner, R. M.; Rudolph, L.; Matrisian, L.; Slayden, Ov.

    In: Human Reproduction, Vol. 11, No. SUPPL. 2, 1996, p. 150-164.

    Research output: Contribution to journalArticle

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