Abstract
Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen-SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen-SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 h treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen-SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen-SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8210-8217 |
| Number of pages | 8 |
| Journal | Biomaterials |
| Volume | 31 |
| Issue number | 32 |
| DOIs | |
| State | Published - Nov 2010 |
Fingerprint
Keywords
- Collagen
- ECM (extracellular matrix)
- Matrix metalloproteinases
- Optical coherence tomography
- Smooth muscle cell
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography. / Levitz, David; Hinds, Monica; Ardeshiri, Ardi; Hanson, Stephen R.; Jacques, Steven.
In: Biomaterials, Vol. 31, No. 32, 11.2010, p. 8210-8217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography
AU - Levitz, David
AU - Hinds, Monica
AU - Ardeshiri, Ardi
AU - Hanson, Stephen R.
AU - Jacques, Steven
PY - 2010/11
Y1 - 2010/11
N2 - Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen-SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen-SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 h treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen-SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen-SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.
AB - Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen-SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen-SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 h treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen-SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen-SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.
KW - Collagen
KW - ECM (extracellular matrix)
KW - Matrix metalloproteinases
KW - Optical coherence tomography
KW - Smooth muscle cell
UR - http://www.scopus.com/inward/record.url?scp=77956187517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956187517&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2010.06.042
DO - 10.1016/j.biomaterials.2010.06.042
M3 - Article
C2 - 20708790
AN - SCOPUS:77956187517
VL - 31
SP - 8210
EP - 8217
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 32
ER -