NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas

Yinhua Yu, Fengji Xu, Hongqi Peng, Xianjun Fang, Shulei Zhao, Yang Li, Bruce Cuevas, Wen Lin Kuo, Joe W. Gray, Michael Siciliano, Gordon B. Mills, Robert C. Bast

Research output: Contribution to journalArticle

253 Scopus citations

Abstract

Using differential display PCR, we have identified a gene [NOEY2, ARHI (designation by the Human Gene Nomenclature Committee)] with high homology to ras and rap that is expressed consistently in normal ovarian and breast epithelial cells but not in ovarian and breast cancers. Reexpression of NOEY2 through transfection suppresses clonogenic growth of breast and ovarian cancer cells. Growth suppression was associated with down-regulation of the cyclin D1 promoter activity and induction of p21(WAF1/CIP1). In an effort to identify mechanisms leading to NOEY2 silencing in cancer, we found that the gene is expressed monoallelically and is imprinted maternally. Loss of heterozygosity of the gene was detected in 41% of ovarian and breast cancers. In most of cancer samples with loss of heterozygosity, the nonimprinted functional allele was deleted. Thus, NOEY2 appears to be a putative imprinted tumor suppressor gene whose function is abrogated in ovarian and breast cancers.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number1
DOIs
StatePublished - Jan 5 1999

ASJC Scopus subject areas

  • General

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