NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1AM treatment

J. A. Haviland, H. Reiland, D. E. Butz, M. Tonelli, W. P. Porter, R. Zucchi, Thomas (Tom) Scanlan, G. Chiellini, F. M. Assadi-Porter

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective 3-Iodothyronamine (T1AM), an analog of thyroid hormone, is a recently discovered fast-acting endogenous metabolite. Single high-dose treatments of T1AM have produced rapid short-term effects, including a reduction of body temperature, bradycardia, and hyperglycemia in mice. Design and Methods The effect of daily low doses of T1AM (10 mg/kg) for 8 days on weight loss and metabolism in spontaneously overweight mice was monitored. The experiments were repeated twice (n = 4). Nuclear magnetic resonance (NMR) spectroscopy of plasma and real-time analysis of exhaled 13CO2 in breath by cavity ring down spectroscopy (CRDS) were used to detect T1AM-induced lipolysis. Results CRDS detected increased lipolysis in breath shortly after T1AM administration that was associated with a significant weight loss but independent of food consumption. NMR spectroscopy revealed alterations in key metabolites in serum: valine, glycine, and 3-hydroxybutyrate, suggesting that the subchronic effects of T1AM include both lipolysis and protein breakdown. After discontinuation of T1AM treatment, mice regained only 1.8% of the lost weight in the following 2 weeks, indicating lasting effects of T 1AM on weight maintenance. Conclusions CRDS in combination with NMR and 13C-metabolic tracing constitute a powerful method of investigation in obesity studies for identifying in vivo biochemical pathway shifts and unanticipated debilitating side effects.

Original languageEnglish (US)
Pages (from-to)2538-2544
Number of pages7
JournalObesity
Volume21
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Metabolomics
Lipolysis
Spectrum Analysis
Magnetic Resonance Spectroscopy
Lipids
Weight Loss
Weights and Measures
Proteins
3-Hydroxybutyric Acid
Valine
Bradycardia
Body Temperature
Thyroid Hormones
Hyperglycemia
Glycine
Obesity
Maintenance
Food
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Haviland, J. A., Reiland, H., Butz, D. E., Tonelli, M., Porter, W. P., Zucchi, R., ... Assadi-Porter, F. M. (2013). NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1AM treatment. Obesity, 21(12), 2538-2544. https://doi.org/10.1002/oby.20391

NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1AM treatment. / Haviland, J. A.; Reiland, H.; Butz, D. E.; Tonelli, M.; Porter, W. P.; Zucchi, R.; Scanlan, Thomas (Tom); Chiellini, G.; Assadi-Porter, F. M.

In: Obesity, Vol. 21, No. 12, 12.2013, p. 2538-2544.

Research output: Contribution to journalArticle

Haviland, JA, Reiland, H, Butz, DE, Tonelli, M, Porter, WP, Zucchi, R, Scanlan, TT, Chiellini, G & Assadi-Porter, FM 2013, 'NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1AM treatment', Obesity, vol. 21, no. 12, pp. 2538-2544. https://doi.org/10.1002/oby.20391
Haviland, J. A. ; Reiland, H. ; Butz, D. E. ; Tonelli, M. ; Porter, W. P. ; Zucchi, R. ; Scanlan, Thomas (Tom) ; Chiellini, G. ; Assadi-Porter, F. M. / NMR-based metabolomics and breath studies show lipid and protein catabolism during low dose chronic T1AM treatment. In: Obesity. 2013 ; Vol. 21, No. 12. pp. 2538-2544.
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abstract = "Objective 3-Iodothyronamine (T1AM), an analog of thyroid hormone, is a recently discovered fast-acting endogenous metabolite. Single high-dose treatments of T1AM have produced rapid short-term effects, including a reduction of body temperature, bradycardia, and hyperglycemia in mice. Design and Methods The effect of daily low doses of T1AM (10 mg/kg) for 8 days on weight loss and metabolism in spontaneously overweight mice was monitored. The experiments were repeated twice (n = 4). Nuclear magnetic resonance (NMR) spectroscopy of plasma and real-time analysis of exhaled 13CO2 in breath by cavity ring down spectroscopy (CRDS) were used to detect T1AM-induced lipolysis. Results CRDS detected increased lipolysis in breath shortly after T1AM administration that was associated with a significant weight loss but independent of food consumption. NMR spectroscopy revealed alterations in key metabolites in serum: valine, glycine, and 3-hydroxybutyrate, suggesting that the subchronic effects of T1AM include both lipolysis and protein breakdown. After discontinuation of T1AM treatment, mice regained only 1.8{\%} of the lost weight in the following 2 weeks, indicating lasting effects of T 1AM on weight maintenance. Conclusions CRDS in combination with NMR and 13C-metabolic tracing constitute a powerful method of investigation in obesity studies for identifying in vivo biochemical pathway shifts and unanticipated debilitating side effects.",
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AU - Porter, W. P.

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AU - Scanlan, Thomas (Tom)

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