NMDA receptors mediate leptin signaling and regulate potassium channel trafficking in pancreatic Β-cells

Yi Wu, Dale A. Fortin, Veronica A. Cochrane, Pei Chun Chen, Show Ling Shyng

Research output: Research - peer-reviewArticle

Abstract

NMDA receptors (NMDARs) are Ca2-permeant, ligand-gated ion channels activated by the excitatory neurotransmitter glutamate and have well-characterized roles in the nervous system. The expression and function of NMDARs in pancreatic -cells, by contrast, are poorly understood. Here, we report a novel function of NMDARs in -cells. Using a combination of biochemistry, electrophysiology, and imaging techniques, we now show that NMDARs have a key role in mediating the effect of leptin to modulate -cell electrical activity by promoting AMP-activated protein kinase (AMPK)-dependent trafficking of KATP and Kv2.1 channels to the plasma membrane. Blocking NMDAR activity inhibited the ability of leptin to activate AMPK, induce KATP and Kv2.1 channel trafficking, and promote membrane hyperpolarization. Conversely, activation of NMDARs mimicked the effect of leptin, causing Ca2 influx, AMPK activation, and increased trafficking of KATP and Kv2.1 channels to the plasma membrane, and triggered membrane hyperpolarization. Moreover, leptin potentiated NMDAR currents and triggered NMDAR-dependent Ca2 influx. Importantly, NMDAR-mediated signaling was observed in rat insulinoma 832/13 cells and in human -cells, indicating that this pathway is conserved across species. The ability of NMDARs to regulate potassium channel surface expression and thus, -cell excitability provides mechanistic insight into the recently reported insulinotropic effects of NMDAR antagonists and therefore highlights the therapeutic potential of these drugs in managing type 2 diabetes.

LanguageEnglish (US)
Pages15512-15524
Number of pages13
JournalJournal of Biological Chemistry
Volume292
Issue number37
DOIs
StatePublished - 2017

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Potassium Channels
Leptin
N-Methyl-D-Aspartate Receptors
AMP-Activated Protein Kinases
Adenosine Triphosphate
Cell membranes
Chemical activation
Membranes
Cell Membrane
Electrophysiology
Ligand-Gated Ion Channels
Biochemistry
Neurology
Medical problems
Neurotransmitter Agents
Rats
Glutamic Acid
Imaging techniques
Pharmaceutical Preparations
Insulinoma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

NMDA receptors mediate leptin signaling and regulate potassium channel trafficking in pancreatic Β-cells. / Wu, Yi; Fortin, Dale A.; Cochrane, Veronica A.; Chen, Pei Chun; Shyng, Show Ling.

In: Journal of Biological Chemistry, Vol. 292, No. 37, 2017, p. 15512-15524.

Research output: Research - peer-reviewArticle

Wu, Yi ; Fortin, Dale A. ; Cochrane, Veronica A. ; Chen, Pei Chun ; Shyng, Show Ling. / NMDA receptors mediate leptin signaling and regulate potassium channel trafficking in pancreatic Β-cells. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 37. pp. 15512-15524
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