NMDA receptor subunit expression in GABAergic interneurons in the prefrontal cortex: Application of laser microdissection technique

Dong Xi, Benjamin Keeler, Wentong Zhang, John D. Houle, Wen Jun Gao

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The selective involvement of a subset of neurons in many psychiatric disorders, such as gamma-aminobutyric acid (GABA)-ergic interneurons in schizophrenia, creates a significant need for in-depth analysis of these cells. Here we introduce a combination of techniques to examine the relative gene expression of N-methyl-d-aspartic acid (NMDA) receptor subtypes in GABAergic interneurons from the rat prefrontal cortex. Neurons were identified by immunostaining, isolated by laser microdissection and RNA was prepared for reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. These experimental procedures have been described individually; however, we found that this combination of techniques is powerful for the analysis of gene expression in individual identified neurons. This approach provides the means to analyze relevant molecular mechanisms that are involved in the neuropathological process of a devastating brain disorder.

Original languageEnglish (US)
Pages (from-to)172-181
Number of pages10
JournalJournal of Neuroscience Methods
Volume176
Issue number2
DOIs
StatePublished - Jan 30 2009
Externally publishedYes

Keywords

  • Laser microdissection
  • NMDA receptors
  • NovaRed
  • Parvalbumin
  • Real-time polymerase chain reaction
  • Schizophrenia

ASJC Scopus subject areas

  • Neuroscience(all)

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