NMDA receptor agonists fail to alter release from cerebellar basket cells

Jason R. Pugh, Craig Jahr

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previous studies ofNMDAreceptor (NMDAR) expression on axons of cerebellar molecular layer interneurons have produced conflicting results. We made use of the calcium sensitivity of vesicular release machinery to test for NMDAR activity in basket cell axons. Iontophoresis of L-aspartate, anNMDARagonist, onto basket cell axon collaterals had no effect on evoked IPSCs measured in synaptically coupled Purkinje cells. Furthermore, calcium indicators in basket cell varicosities did not report any change in intracellular calcium following iontophoresis of L-aspartate or two-photon uncaging of glutamate. In contrast, activation of presynaptic purinergic receptors by iontophoresis of ATP decreased evoked IPSC amplitudes and action potential-evoked calcium transients in axonal varicosities, demonstrating the effectiveness of activating presynaptic receptors by iontophoresis. We find no evidence for functional NMDARs in basket cell varicosities.

Original languageEnglish (US)
Pages (from-to)16550-16555
Number of pages6
JournalJournal of Neuroscience
Volume31
Issue number46
DOIs
StatePublished - Nov 16 2011

Fingerprint

Iontophoresis
N-Methyl-D-Aspartate Receptors
Calcium
Presynaptic Receptors
Axons
Aspartic Acid
Purinergic Receptors
Purkinje Cells
Interneurons
Photons
Action Potentials
Glutamic Acid
Adenosine Triphosphate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

NMDA receptor agonists fail to alter release from cerebellar basket cells. / Pugh, Jason R.; Jahr, Craig.

In: Journal of Neuroscience, Vol. 31, No. 46, 16.11.2011, p. 16550-16555.

Research output: Contribution to journalArticle

Pugh, Jason R. ; Jahr, Craig. / NMDA receptor agonists fail to alter release from cerebellar basket cells. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 46. pp. 16550-16555.
@article{187f3b5e1c864f03884f9b83f0813d60,
title = "NMDA receptor agonists fail to alter release from cerebellar basket cells",
abstract = "Previous studies ofNMDAreceptor (NMDAR) expression on axons of cerebellar molecular layer interneurons have produced conflicting results. We made use of the calcium sensitivity of vesicular release machinery to test for NMDAR activity in basket cell axons. Iontophoresis of L-aspartate, anNMDARagonist, onto basket cell axon collaterals had no effect on evoked IPSCs measured in synaptically coupled Purkinje cells. Furthermore, calcium indicators in basket cell varicosities did not report any change in intracellular calcium following iontophoresis of L-aspartate or two-photon uncaging of glutamate. In contrast, activation of presynaptic purinergic receptors by iontophoresis of ATP decreased evoked IPSC amplitudes and action potential-evoked calcium transients in axonal varicosities, demonstrating the effectiveness of activating presynaptic receptors by iontophoresis. We find no evidence for functional NMDARs in basket cell varicosities.",
author = "Pugh, {Jason R.} and Craig Jahr",
year = "2011",
month = "11",
day = "16",
doi = "10.1523/JNEUROSCI.3910-11.2011",
language = "English (US)",
volume = "31",
pages = "16550--16555",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "46",

}

TY - JOUR

T1 - NMDA receptor agonists fail to alter release from cerebellar basket cells

AU - Pugh, Jason R.

AU - Jahr, Craig

PY - 2011/11/16

Y1 - 2011/11/16

N2 - Previous studies ofNMDAreceptor (NMDAR) expression on axons of cerebellar molecular layer interneurons have produced conflicting results. We made use of the calcium sensitivity of vesicular release machinery to test for NMDAR activity in basket cell axons. Iontophoresis of L-aspartate, anNMDARagonist, onto basket cell axon collaterals had no effect on evoked IPSCs measured in synaptically coupled Purkinje cells. Furthermore, calcium indicators in basket cell varicosities did not report any change in intracellular calcium following iontophoresis of L-aspartate or two-photon uncaging of glutamate. In contrast, activation of presynaptic purinergic receptors by iontophoresis of ATP decreased evoked IPSC amplitudes and action potential-evoked calcium transients in axonal varicosities, demonstrating the effectiveness of activating presynaptic receptors by iontophoresis. We find no evidence for functional NMDARs in basket cell varicosities.

AB - Previous studies ofNMDAreceptor (NMDAR) expression on axons of cerebellar molecular layer interneurons have produced conflicting results. We made use of the calcium sensitivity of vesicular release machinery to test for NMDAR activity in basket cell axons. Iontophoresis of L-aspartate, anNMDARagonist, onto basket cell axon collaterals had no effect on evoked IPSCs measured in synaptically coupled Purkinje cells. Furthermore, calcium indicators in basket cell varicosities did not report any change in intracellular calcium following iontophoresis of L-aspartate or two-photon uncaging of glutamate. In contrast, activation of presynaptic purinergic receptors by iontophoresis of ATP decreased evoked IPSC amplitudes and action potential-evoked calcium transients in axonal varicosities, demonstrating the effectiveness of activating presynaptic receptors by iontophoresis. We find no evidence for functional NMDARs in basket cell varicosities.

UR - http://www.scopus.com/inward/record.url?scp=81255211984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81255211984&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.3910-11.2011

DO - 10.1523/JNEUROSCI.3910-11.2011

M3 - Article

VL - 31

SP - 16550

EP - 16555

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 46

ER -